実験用フッ化物ワニス中の接着成分が 30 日以内のフッ化物徐放に及ぼす影響 in vitro 試験

Effect of adhesive components in experimental fluoride varnish on fluoride release within 30 days in vitro study.

抄録

ウシ歯に塗布した実験用フッ化物ワニスからの有効フッ化物濃度の放出時間を接着成分が増加させるか否かを検討することを目的とした。5%フッ化ナトリウムを含む実験用フッ化物ワニス(EX1)を調製し，35%ヒドロキシエチルメタクリレート(HEMA)(EX2)，5%グルタルアルデヒド(EX3)または35%HEMA/5%グルタルアルデヒド混合物(EX4)と接着させた．比較のために2種類の市販フッ素ワニスを用いた。各群を牛切歯に塗布し、30日間フッ素徐放性およびpHをモニターした。細胞生存率分析、走査型電子顕微鏡、エネルギー分散型分光法を行った。EX4は最も高い濃度のフッ化物を最も長い期間放出し、最も早く中性pHに達した。結論として、EX4はう蝕予防に最も効果的なワニスであると考えられる。

We aimed to determine whether adhesive components could increase the release time of effective fluoride concentration from an experimental fluoride varnish applied to bovine teeth. An experimental fluoride varnish containing 5% sodium fluoride (EX1) was prepared and combined with 35% hydroxyethyl methacrylate (HEMA) (EX2), 5% glutaraldehyde (EX3), or 35% HEMA/5% glutaraldehyde mixture (EX4). Two commercially available fluoride varnishes were used for comparison. Each group was applied to bovine incisors, and the fluoride release and pH were monitored for 30 days. Cell viability analysis, scanning electron microscopy, and energy-dispersive spectroscopy were performed. EX4 released the highest and most effective concentration of fluoride for the longest period and reached neutral pH at the earliest; the release was maintained for up to 30 days without cytotoxicity. In conclusion, EX4 is considered to be the most effective varnish to prevent dental caries.