根管からのEnterococcus faecalisの減少におけるWaveOne GoldとOne Shape New Generationの評価

Evaluation of the WaveOne Gold and One Shape New Generation in Reducing Enterococcus faecalis from Root Canal.

抄録

本研究の目的は，Enterococcus faecalisに感染した根管からの細菌除去に関して，WaveOne GoldおよびOne Shape New Generationシステムを従来のWaveOneおよびOne Shapeシステムと比較し，評価することであった．エチレンオキサイド滅菌した上顎臼歯部遠心根管48本をE. faecalisに21日間感染させ，根管初期細菌をペーパーコーンで採取し，M-enterococcus寒天培地でプレーティングした．検体はインスツルメンテーションによりランダムに4群に分けた．WaveOne Gold，One Shape New Generation，WaveOne，One Shapeの4群に無作為に分けた．インスツルメンテーション後，インスツルメンテーション直後と7日目にスクレイピングとペーパーコーンを用いて検体を採取した．細菌の減少量を計算し、Friedman testによるグループ内分析、Kruskal-WallisとDunnのpost-hoc testによるグループ間分析を行い、いずれも5％の有意水準で行った。どの手法も根管内の細菌数を有意に減少させた（p<0.05）。WaveOne GoldとOne Shape New GenerationはWaveOneとOne Shapeシステムよりも高い細菌減少を促進したが（p<0.05）、WaveOne GoldとOne Shape New Generation間、WaveOneとOne Shape間に有意差は認められなかった（p>0.05）。新規シングルファイルシステムは、従来のシングルファイルシステムに比べ、より優れた細菌除去を促進する。

The aim of this study was to evaluate the WaveOne Gold and One Shape New Generation systems regarding the bacterial removal from root canals infected with Enterococcus faecalis by comparing them to the conventional WaveOne and One Shape systems. Forty-eight distobuccal root canals of maxillary molars sterilized with ethylene oxide were infected with E. faecalis for 21 days, and then root canal initial bacterial sample was collected with paper cones and plated on M-enterococcus agar. The specimens were randomly divided into 4 groups according to the instrumentation: WaveOne Gold, One Shape New Generation, WaveOne and One Shape. After instrumentation, samples were collected with use of scraping and paper cones at immediate and 7 days after instrumentation. The bacterial reduction was calculated and then made intragroup analysis by Friedman test and intergroup analysis by Kruskal-Wallis with Dunn's post-hoc test, all at 5% significance. All techniques significantly reduced the number of bacteria in the root canal (p<0.05). WaveOne Gold and One Shape New Generation promoted higher bacterial reduction than WaveOne and One Shape systems (p<0.05), but no significant difference was found between WaveOne Gold and One Shape New Generation or between WaveOne and One Shape (p>0.05). Novel single-file systems promote better bacterial removal than the conventional single-file systems.