鉄炭素をベースとした建設湿地による都市廃水処理のための微生物種間相互作用と窒素代謝経路の解明

Microbial interspecific interaction and nitrogen metabolism pathway for the treatment of municipal wastewater by iron carbon based constructed wetland.

• Xiao Huang
• Xinmei Yang
• Jia Zhu
• Jianghua Yu

抄録

本研究では，鉄-Cフィラーを用いた構築湿地(CW)における汚染物質除去性能と種間相互作用を調べるために，鉄-Cフィラーを用いた構築湿地(CW-Fe)とセラムサイトフィラーを用いた構築湿地(CW-C)を設置した．その結果，炭素源を含まないCW-Fe系では，高分子有機物を高分子有機物に変換することができたが，全窒素の除去効率はCW-C系に比べて低いことがわかった．しかし、アンモニア性窒素(NH-N)の増加は、全リン(TP)除去性能の高いCW-Fe系で観察された。ハイスループットシーケンシングの結果、Fe-C充填材を用いた場合、セラムサイト充填材を用いた場合に比べて、Bacteroides, Firmicutes, Chlorofeli, Actinobacteriaの微生物の豊富さと豊富さが有意に高くなることがわかった。また、2つのCW間の相互作用は有意に異なり、CW-Fe系の硝酸態窒素(NO-N)からNH-Nへの変換の機能性酵素の豊富さは有意に増加した。

In order to explore the pollutant removal performance and interspecific interaction in constructed wetland (CW) with Fe-C filler, constructed wetland with Fe-C filler (CW-Fe) and with ceramsite filler (CW-C) were set up. Besides, the nutrients removal and interspecific interaction were analyzed, and the results showed that total nitrogen (TN) removal efficiency of CW-Fe system without carbon source was lower than that in CW-C system though CW-Fe system could convert macro-molecular organic matter into micro-molecular organic matter. However, ammonia nitrogen (NH-N) increase was observed in CW-Fe system with better total phosphorus (TP) removal performance. High-throughput sequencing showed that the microbial richness and abundance of Bacteroides, Firmicutes, Chlorofeli and Actinobacteria in the CW with Fe-C filler was significantly higher than with ceramsite filler. The interaction between two CWs was significantly different, and the functional enzymes abundance of nitrate nitrogen (NO-N) to NH-N transformation in CW-Fe system significantly increased.

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