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Rhinopithecus roxellanaeの糞便から分離されたFalsigemobacter faecalis gen.nov
Falsigemmobacter faecalis gen. nov. sp. nov., isolated from faeces of Rhinopithecus roxellanae, and reclassification of Gemmobacter intermedius as Falsigemmobacter intermedius comb. nov.
PMID: 32686033 DOI: 10.1007/s00203-020-01978-w.
抄録
中国雲南省雲南野生動物公園で採取したRhinopithecus roxellanaeの糞便から分離した。細胞はグラム染色陰性、好気性、非増殖性、不規則な棒状であった。最適な増殖は、0-1.0% (w/v) NaCl、pH7.0-8.0、30℃で行われた。ユビキノンはQ-10が優勢であった。主要な細胞性脂肪酸（>10.0%）はCω7cとCω7c/Cω6cであった。支配的な極性脂質は、ジホスファチジルグリセロール、ホスファチジルグリセロール、ホスファチジルエタノールアミン、ホスファチジルコリンであった。DNA G+C含量は62.4mol%であった。16S rRNA遺伝子配列に基づく系統解析の結果、YIM 102744-1はRhodobacteraceaeに属し、型菌Gemmobacter intermedius 119/4と最も高い類似度を共有していた（96.6％）。また，系統樹からは，YIM 102744-1株は近縁のG. intermedius 119/4株とは異なる系統樹を形成していることが示唆された．以上の結果から，YIM 102744-1株はFalsigemobacter属に属する新種であると考えられ，Falsigemobacter faecalis gen.Falsigemmobacter faecalisの型株はYIM 102744-1(=KCTC 52106=CCTCC AB 2016031)である。Gemmobacter intermedius 119/4は系統樹上でYIM 102744-1と分岐を形成し、Gemmobacter属内で他のメンバーと明確に分離されていたことから、Falsigemobacter intermedius comb.nov.としてFalsigemobacter属に移行することも提案されている。型株119/4=CIP 110795=LMG 28215=CCM 8510）としてFalsigemobacter属に編入することも提案されている。Falsigemmobacter属の型は、Falsigemmobacter intermedius gen.nov.
Strain YIM 102744-1 was isolated from Rhinopithecus roxellanae fecal sample collected at Yunnan Wild Animal Park, Yunnan province, PR China. Cells were Gram-stain-negative, aerobic, non-motile and irregular rods. Optimal growth occurred in the presence of 0-1.0% (w/v) NaCl, at pH 7.0-8.0, and at 30 °C. The predominant ubiquinone was Q-10. The major cellular fatty acids (> 10.0%) were Cω7c and Cω7c/Cω6c. The dominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine. The DNA G + C content was 62.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that YIM 102744-1 belonged to the family Rhodobacteraceae and shared the highest similarity with the type strain Gemmobacter intermedius 119/4 (96.6%). In addition, phylogenetic trees indicated that strain YIM 102744-1 formed a distinct clade together with the closest relative G. intermedius 119/4. Based on the results of polyphasic taxonomic analysis, strain YIM 102744-1 is considered to represent a novel species within a new genus Falsigemmobacter, for which the name Falsigemmobacter faecalis gen. nov., sp. nov. is proposed. The type strain of Falsigemmobacter faecalis is YIM 102744-1(= KCTC 52106 = CCTCC AB 2016031). Because Gemmobacter intermedius 119/4 formed a branch with YIM 102744-1 in the phylogenetic trees and was clearly separated from the other members within the genus Gemmobacter, it is also proposed to transfer into the genus Falsigemmobacter as Falsigemmobacter intermedius comb. nov. (type strain 119/4 = CIP 110795 = LMG 28215 = CCM 8510). The type species of the genus Falsigemmobacter is Falsigemmobacter intermedius gen. nov., comb. nov.