歯科用接着剤である4-META/MMA-TBB樹脂が再生口腔上皮の接着および角化に及ぼす影響

Effect of the dental adhesive, 4-META/MMA-TBB resin, on adhesion and keratinization of regenerating oral epithelium.

抄録

背景と目的:

4-META/MMA-TBB [4-(2-methacryloxyethyl)trimellitic anhydride/methyl methacrylate-tributylborane] 樹脂は歯科用接着剤として広く使用されている．また，歯周外科手術後の歯肉創傷面のドレッシングにも応用されている．しかし，口腔内上皮の再生や細胞接着に及ぼす影響については未解明である．そこで、本樹脂を創傷被覆材として適用し、歯肉切除術後の再生口腔上皮における laminin 5, integrin beta(4) および cytokeratin 14 の発現を細胞接着および分化の観点から検討した。

BACKGROUND AND OBJECTIVE: The 4-META/MMA-TBB [4-(2-methacryloxyethyl)trimellitic anhydride/methyl methacrylate-tributylborane] resin is widely used as a dental adhesive. It has also been applied in the dressing of gingival wound surfaces following periodontal surgery. However, its effect on the regeneration and/or cell attachment of the oral epithelium remains to be clarified. To evaluate the effect of the resin applied as a wound dressing, we investigated expression of laminin 5, integrin beta(4) and cytokeratin 14 in regenerating oral epithelium treated with this resin following gingivectomy from the viewpoint of cell attachment and differentiation.

材料および方法:

ラットの歯肉切除術後の創傷面全体に本樹脂を塗布し、その直後と1、3、5、7、14日後に再生上皮を観察した。一部の動物では塗布後2週間目にレジンを除去し、さらに1、3、5、7日後に組織を観察した。

MATERIAL AND METHODS: The resin was applied to the entire wound surface in rats after gingival surgery, and regenerating epithelium was examined immediately and at 1, 3, 5, 7 and 14 days later. The resin was removed 2 weeks after application in some animals and tissue further examined at 1, 3, 5 and 7 days later.

結果:

レジン下の再生上皮は角化していなかったが、レジン除去後すぐに角化した。Laminin 5 と integrin beta(4) は基底膜、内部基底膜、再生上皮の辺縁細胞、樹脂-再生上皮界面に免疫局在していた。Cytokeratin 14は、レジン下の再生上皮、健常上皮、再生上皮の接合部上皮細胞に局在していた。

RESULTS: Regenerating epithelium under the resin was not keratinized, but became keratinized immediately after removal of the resin. Laminin 5 and integrin beta(4) were immunolocalized in the basal lamina, the internal basal lamina, in marginal cells of the regenerating epithelium and at the resin-regenerating epithelium interface. Cytokeratin 14 localized in the regenerating epithelium underneath the resin, as well as in healthy and regenerated junctional epithelial cells.

結論:

これらの結果は、この樹脂が創傷面を覆い、その再生の初期過程で再生上皮が樹脂に生物学的に付着していることを示唆している。

CONCLUSION: These results suggest that this resin covers the wound surface and that the regenerating epithelium biologically adheres to the resin during the initial process of its regeneration.