内面漂白後の歯髄室から外根面への過酸化物の浸透について

Peroxide penetration from the pulp chamber to the external root surface after internal bleaching.

抄録

目的:

ウォーキングブリーチングの際に歯髄室から歯の外表面に浸透する過酸化物の量を定量化すること。

PURPOSE: To quantify the amount of peroxide penetration from the pulp chamber to the external surface of teeth during the walking bleaching technique.

方法:

牛側切歯 72 本を 5 つの実験群と 1 つの対照群に無作為に分けた（各群 n = 12）：(1)35%過酸化水素 (HP); (2)35% 過酸化カルバミド (CP); (3)sodium perborate (SP); (4) (HP+SP); (5) (CP+SP) および (6)Control (CG) （脱イオン水）.全グループともウォーキングブリーチ法に準じて処理した。酢酸緩衝液中37℃で7日後、100μlのバイオレット・ロイコクリスタル着色料と50μlのペルオキシダーゼを加え、分光光度計で測定できる青色の染色を生成し、過酸化物μg/mlに変換した。

METHODS: Seventy-two bovine lateral incisors were randomly divided over five experimental groups and one control (n = 12 per group): (1) 35% hydrogen peroxide (HP); (2) 35% carbamide peroxide (CP); (3) sodium perborate (SP); (4) (HP+SP); (5) (CP+SP) and (6) Control (CG), deionized water. All groups were treated according to the walking bleach technique. After 7 days at 37 degrees C in an acetate buffer solution, 100 microl violet leukocrystal coloring and 50 microl peroxidase was added, producing a blue stain that could be measured in a spectrophotometer and then converted into peroxide microg/ml.

結果:

G5が最も浸透性が高く、G2およびG3が最も低い値を示した。すべての漂白剤が歯髄室から外歯根面まで浸透した．酸化剤の存在と浸透性には直接的な相関が認められた．蒸留水中の過ホウ酸ナトリウムは酸化力が弱く，最も攻撃的でない漂白剤と考えられた．

RESULTS: G5 exhibited the greatest penetration, while G2 and G3 produced the lowest values. All bleaching agents penetrated from the pulp chamber to the external root surface. There was a direct correlation between the presence of oxidative agents and penetration potential. Sodium perborate in distilled water was less oxidative and appeared to be the least aggressive bleaching agent.