モルモットにおける選択された血液炎症性バイオマーカーに対する鉛曝露の影響

The effect of lead exposure on selected blood inflammatory biomarkers in guinea pigs.

• Tahere Farkhondeh
• Mohammad Hossein Boskabady
• Mohammd Kazem Koohi
• Goudarz Sadeghi-Hashjin
• Mostafa Moin

抄録

本研究の目的は、感作動物と比較してモルモットの血中炎症性バイオマーカーに対する酢酸鉛の影響を調べることであった。モルモット30頭を対照群(C群)、感作群(S群)、3群(0.1M Pb, 0.2M Pb, 0.4M Pb)に無作為に割り付けた。エアロゾル化オバルブミン（OA）チャレンジ後に感作された動物。Pb暴露群は0.1M、0.2Mおよび0.4Mの酢酸鉛を1時間、週3回、2週間吸入した。すべての動物群の血液中の総白血球数および差動白血球数、PLA2活性および総タンパク質レベルを評価した。感作動物と鉛曝露動物の血清PLA2活性、総タンパク質、総WBC数は対照群と比較して有意に高かった（p＜0.05）。対照群と比較して、好酸球、ニュートロフィル、好塩基球の割合も増加した（p <0.01）。また、高濃度の鉛に曝露された動物では好酸球と好塩基球の割合が、中濃度の鉛に曝露された動物では好塩基球の割合が対照群と比較して有意に増加した(p <0.05)。また、対照群との間では、濃度依存的にリンパ球の割合が減少していた。以上のことから、酢酸鉛の吸入暴露は喘息様疾患を引き起こす可能性があることが示唆された。

This study was aimed to examine the effect of lead acetate on inflammatory biomarkers in blood of guinea pigs in comparison with sensitized animals. Thirty guinea pigs were randomly allocated into control (group C), sensitized (group S), and three Pb-exposed (groups 0.1M Pb, 0.2M Pb and 0.4M Pb). Animal sensitized after aerosolized-ovalbumin (OA) challenge. Pb-exposed groups inhaled 0.1M, 0.2M and 0.4M lead acetate for 1 h, three times a week for two weeks. Total and differential WBC counts, PLA2 activity and total protein levels were evaluated in blood of all animal groups. Serum PLA2 activity, total protein and total WBC number in sensitized and animal exposed to lead were significantly higher as compared to control group (p <0.05). When compared to control group, the percentages of eosinophil, nuetrophil and basophiles were also increased in sensitized guinea pigs (p <0.01). The percentage of eosinophil and basophile in animals exposed to high level of lead and that of basophile in animals exposed to middle lead concentration versus to control group significantly increased (p <0.05). In addition, the significant difference between the lymphocyte percentages of Pb-exposed groups and control group were decreased in concentration dependent manner. The findings of the present study indicated that exposure to inhaled lead acetate may lead to asthma-like disease.