verruga peruanaの2人の患者の血液から分離されたBartonella ancashensis sp.nov.の説明

Description of Bartonella ancashensis sp. nov., isolated from the blood of two patients with verruga peruana.

• Kristin E Mullins
• Jun Hang
• Ju Jiang
• Mariana Leguia
• Matthew R Kasper
• Palmira Ventosilla
• Ciro Maguiña
• Richard G Jarman
• David Blazes
• Allen L Richards

抄録

ペルーのアンカシュ州カラズで慢性期のBartonella bacilliformis感染症（verruga peruana）の治療を目的とした臨床試験に登録された2名の患者の血液から，Bartonella属の新規分離株3株が分離された．分離された菌は，最初にgltA遺伝子の断片の塩基配列を解析したところ，B. bacilliformisとは異なることが判明した．さらに、表現型および遺伝子型解析法を用いて分離株の特徴を調べたところ、遺伝子的には互いに同一であるが、近縁種のB. bacilliformisを含むBartonella属の既知の種とは異なることが判明した。また，分離株の形態，顕微鏡的・生化学的特性，生育パターンなどの他の特徴は，Bartonella属と一致していた。以上の結果から，これら3株はBartonella属の新種であり，Bartonella ancashensis sp.20.00T=ATCC BAA-2694T=DSM 29364T）と命名した。

Three novel isolates of the genus Bartonella were recovered from the blood of two patients enrolled in a clinical trial for the treatment of chronic stage Bartonella bacilliformis infection (verruga peruana) in Caraz, Ancash, Peru. The isolates were initially characterized by sequencing a fragment of the gltA gene, and found to be disparate from B. bacilliformis. The isolates were further characterized using phenotypic and genotypic methods, and found to be genetically identical to each other for the genes assessed, but distinct from any known species of the genus Bartonella, including the closest relative B. bacilliformis. Other characteristics of the isolates, including their morphology, microscopic and biochemical properties, and growth patterns, were consistent with members of the genus Bartonella. Based on these results, we conclude that these three isolates are members of a novel species of the genus Bartonella for which we propose the name Bartonella ancashensis sp. nov. (type strain 20.00T = ATCC BAA-2694T = DSM 29364T).