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乳癌MDA-MB-231およびMCF-7細胞株におけるCD44/CD24に対する常温マイクロ波照射の効果
Effects of normothermic microwave irradiation on CD44/CD24 in breast cancer MDA-MB-231 and MCF-7 cell lines.
PMID: 31559912 DOI: 10.1080/09168451.2019.1670044.
抄録
乳がん細胞株であり、がん・がん化細胞（CICs）を有する MDA-MB-231 細胞および MCF-7 細胞が、細胞温度を 37℃に維持した常温マイクロ波照射により死滅したことを報告している。本研究では、MDA-MB-231細胞及びMCF-7細胞のうちCICと定義されたCD44/CD24細胞及びその他の細胞のうち、マイクロ波照射後の生死細胞の割合を調べました。MDA-MB-231細胞のうちCD44/CD24細胞は死滅して細胞数が減少したが、生きたCD44/CD24 MCF-7細胞はマイクロ波照射により増加した。また、MDA-MB-231細胞では接着、浸潤、遊走が減少し、MDA-MB-231細胞ではマトリックスメタロプロテアーゼ2（MMP-2）の活性化がマイクロ波照射により増加した。これらの細胞活性の低下は、MMP-2の活性化とMDA-MB-231細胞のCD44/CD24の母集団の変化によるものと考えられる。APC：アロフェコシアニン；CBB：クーマシーブリリアントブルー；CD：分化クラスター；CICs：がん化細胞；FACS：蛍光活性化細胞ソーティング；FBS：ウシ胎児血清；FITC：フルオレセインイソチオシアネート；FTDT：有限差分時間領域；HER2：ヒト上皮成長因子受容体2型；PI：ヨウ化プロピジウム。
We previously reported that MDA-MB-231 and MCF-7 cells, which are breast cancer cell lines and have cancer and cancer-initiating cells (CICs), were killed following normothermic microwave irradiation in which the cellular temperature was maintained at 37°C. In this study, we investigated the percentages of live or dead cells among CD44/CD24 cells, which were defined as CICs among MDA-MB-231 and MCF-7 cells, and other types of cells in response to microwave irradiation. CD44/CD24 cells among MDA-MB-231 cells were killed, thereby decreasing the number of cells, whereas the number of live CD44/CD24 MCF-7 cells was increased following microwave irradiation. Moreover, adhesion, invasion, and migration were decreased in MDA-MB-231 cells, and the activation of matrix metalloproteinase-2 (MMP-2) in MDA-MB-231 cells was increased following microwave irradiation. These decreased cell activities might have been caused by MMP-2 activation and population changes in CD44/CD24 in MDA-MB-231 cells. APC: allophecocyanin; CBB: coomassie Brilliant Blue; CD: cluster of differentiation; CICs: cancer-initiating cells; FACS: fluorescence-activated cell sorting; FBS: fetal bovine serum; FITC: fluorescein isothiocyanate; FTDT: finite-difference time domain; HER2: human epidermal growth factor receptor type 2; PI: propidium iodide.