ヤギ免疫グロブリン軽鎖遺伝子座における組織と多様性機構の解析

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Immunobiology.2020 Mar;225(2):151889. S0171-2985(19)30319-5. doi: 10.1016/j.imbio.2019.11.024.Epub 2019-11-30.

ヤギ免疫グロブリン軽鎖遺伝子座における組織と多様性機構の解析

The analysis of organization and diversity mechanism in goat immunoglobulin light chain gene loci.

Xiaohui Yu
Lijuan Du
Mingli Wu
Jie Wu
Shuai He
Tingting Yuan
Xiuzhu Sun

PMID: 31812342 DOI: 10.1016/j.imbio.2019.11.024.

抄録

The genomic organization of goat immunoglobulin light chains (Igλ and Igκ) loci were annotated based on the goat genome database. The goat Igλ chain located on chromosome 17 contains at least 35 V gene fragments (seven potential functional genes, one ORF and 27 pseudogenes), two J-C clusters arranged in a V-J2-C1-J1-C2 pattern, with another C3 on scaffold. The Igκ locus included 11 Vκ (five potential functional genes, two ORFs and four pseudogene fragments), three J genes and a single C gene ordered in V-J-C pattern on chromosome 11. By analyzing the clonies of Igλ and Igκ, we further found V2 (26.23 %) &V3 (73.11 %), V2 (52.07 %) &V4 (46.15 %) were predominately used in the expression of λ and κ chains respectively. λ chain showed more abundance in connective diversity than κ chain. Besides, somatic hypermutation with higher frequency in both immunoglobulin light chains was the major mechanism for the goat repertoire diversity. These results demonstrated goat immunoglobulin light chain variable region genome loci and repertoire diversity.