非小細胞肺癌患者におけるマウスダブルミニッツ2ホモログとP73の発現は成長停止DNA損傷誘導遺伝子45αとは相関がなかった：STROBE準拠の研究

The expression of mouse double minute 2 homolog and P73 had no correlation with growth arrest DNA damage-inducible gene 45α in patients with non-small-cell lung carcinoma: A STROBE-compliant study.

• Bo Wang
• Chaoyang Liang
• Huifeng Liu
• Jixing Lin
• Bailin Wang
• Kaijie Fan
• Zhipeng Ren
• Bin Wang
• Tong Li
• Kang Qi
• Xiaodong Tian

抄録

非小細胞肺がん（NSCLC）患者のがん組織とがん隣接組織における成長停止DNA損傷誘導遺伝子45α（GADD45α）、マウス二重分2ホモログ（MDM2）、P73のメッセンジャーリボ核酸（mRNA）およびタンパク質発現の違いを調べるために、GADD45αとMDM2のmRNA発現とタンパク質発現を比較しました。NSCLC患者の肺がんおよびがん隣接組織におけるGADD45αとMDM2のmRNA発現と、GADD45α、MDM2、P73のタンパク質発現を、定量的リアルタイムPCR、免疫組織化学（IHC）、ウェスタンブロット（WB）により比較した。病理型の異なるNSCLC患者におけるGADD45α、MDM2、P73の発現を解析し、これらの遺伝子と性別、喫煙歴、TNM/T病期との相関を解析した。また、WBの結果、腺がん患者のがん組織ではP73タンパク質の発現が有意に減少したが（P"Zs_200A"="Zs_200A".03）、扁平上皮がんでは有意に減少しなかったことが示唆された。NSCLC患者のがん組織とがん隣接組織では、GADD45αの発現に有意な差は認められなかった。

To investigate the difference in messenger ribonucleic acid (mRNA) and protein expression of growth arrest DNA damage-inducible gene 45α (GADD45α), mouse double minute 2 homolog (MDM2), and P73 in cancer and cancer-adjacent tissues in patients with non-small-cell lung carcinoma (NSCLC).We compared the mRNA expression of GADD45α and MDM2 and the protein expression of GADD45α, MDM2, and P73 in lung cancer and cancer-adjacent tissues in NSCLC patients by quantitative real-time PCR, immunohistochemistry (IHC), and Western Blot (WB). We analyzed GADD45α, MDM2, and P73 expression in patients with different pathological types of NSCLC, and the correlation of these genes with gender, smoking history, and TNM/T stages.IHC results suggested that MDM2 protein expression significantly increased in cancer tissues in female patients (P = .01), but not in male patients. In addition, WB results indicated that P73 protein expression significantly decreased in cancer tissues in patients with adenocarcinoma (P = .03), but not squamous carcinoma.MDM2 and P73 protein levels were differentially regulated in cancer and cancer-adjacient tissues in patients with sub types of NSCLC. There was no significant difference in GADD45α expression between cancer and cancer-adjacent tissues in NSCLC patients.