炭化水素で汚染された地下水から分離された新規酵母種とヒト感染症から分離された酵母種

. sp. nov., a novel yeast species isolated from subsoil groundwater contaminated with hydrocarbons and from a human infection.

• Carmela Belloch
• Ana Isabel Pelaez
• Jesús Sánchez
• Cletus P Kurtzman

抄録

スペイン北部の金属加工工場の炭化水素で汚染された地下水中の地下水と米国のヒト感染症から，新種のアナモルフィック酵母に属する酵母株を分離した．分離株間のITS配列を比較したところ、スペイン分離株では0.2"Zs_200A"%の乖離、米国分離株では0.46"Zs_200A"%の乖離が認められた。LSU rRNA遺伝子のD1/D2ドメインに基づいて系統解析を行った結果、これらの分離株は、LSU rRNA遺伝子を持つクラッドおよびその近親者としてクラッドに属することが判明しました。新規分離株との塩基配列の乖離率はそれぞれ1.97および1.79"Zs_200A"%であった。新種の分離株は発酵性がなく，偽菌糸体も産生されなかった。性生殖は個々の分離株および分離株の混合物では観察されなかった。また，本菌と近親者との共役関係は認められなかった。以上の結果から，CECT 12028をホロタイプとする新種の提案がなされた。

Yeast strains belonging to a novel anamorphic yeast species were isolated from subsoil groundwater contaminated with hydrocarbons in a metal working factory located in northern Spain, and from a human infection in the USA. Comparison of ITS sequences between the isolates revealed 0.2 % divergence between the Spanish isolates and 0.46 % divergence between those and the USA isolate. Phylogenetic analysis based on the D1/D2 domains of the LSU rRNA gene showed that these isolates belong to the clade with and as their closest relatives. Sequence divergence between the new isolates and and was 1.97 and 1.79 %, respectively. The isolates in the novel species are not fermentative and pseudohyphae were not produced. Sexual reproduction was not observed for individual isolates or in mixtures of isolates. Conjugation between the isolates in the novel species and close relatives and was not observed. These data support the proposal of as a novel species, with CECT 12028 as the holotype.