ウシ上皮細胞との相互作用は、セロバーに特異的な関連性と侵入パターンを示している

Interaction of with Bovine Epithelial Cells Demonstrates Serovar-Specific Association and Invasion Patterns.

• Serajus Salaheen
• Jakeitha Sonnier
• Seon Woo Kim
• Bradd J Haley
• Jo Ann S Van Kessel

抄録

乳牛はサルモネラ症の感染源として知られており、ヒトサルモネラ症は汚染された乳製品や肉製品、家禽肉や卵の消費に起因しています。多くの血清種が牛の消化管をコロニー化することが知られていますが、乳牛のコメンサルモネラ菌（または持続性）と一過性の血清種と牛の上皮細胞との相互作用はよくわかっていません。ウシ由来の 26 株（Anatum, Cerro, Dublin, Give, Kentucky, Mbandaka, Meleagridis, Montevideo, Muenster, Newport, Oranienburg, Senftenberg, および Typhimurium）のウシ上皮細胞との相互作用の特徴を調べるために、協会侵入アッセイを行った。ウシ上皮細胞との関連性およびウシ上皮細胞の浸潤には、血清種内および血清種間で有意差が認められた（Tukey's Honestly Significant Difference test、<0.05）。また、半固形培地上での運動性についても、異なるセロバ-の株間で有意な差が観察された。本研究から得られた知見は、血清種内および血清種間での菌株間の表現型の多様性が十分に評価されていないことを示しており、今後の研究のベースラインとなるものであり、無症候性キャリーおよびウシサルモネラ症の分子メカニズムを明らかにする可能性があります。

Dairy cows are known reservoirs of and human salmonellosis has been attributed to the consumption of contaminated dairy and beef products as well as poultry meat and eggs. Although many serovars are known to colonize the gastrointestinal tract of cattle, the interactions between dairy commensal (or persistent) and transient serovars with bovine epithelial cells are not well understood. Association-invasion assays were used to characterize the interactions of 26 strains from bovine origins, comprising serovars Anatum, Cerro, Dublin, Give, Kentucky, Mbandaka, Meleagridis, Montevideo, Muenster, Newport, Oranienburg, Senftenberg, and Typhimurium, with cultured bovine epithelial cells. There were significant differences in the association with and invasion of bovine epithelial cells within and across serovars (Tukey's Honestly Significant Difference test,  < 0.05). serovar Dublin strains were the most invasive, whereas Kentucky, Mbandaka, Cerro, and Give strains were the least invasive ( < 0.05). Significant differences in motility on semisolid medium were also observed between strains from different serovars. Findings from this study demonstrate an underappreciated level of phenotypic diversity among strains within and across serovars and serve as a baseline for future studies that may identify the molecular mechanisms of asymptomatic carriage and bovine salmonellosis.