Kolavironは、ラットの母親の剥奪に関連付けられている認知障害とコルチコ-海馬摂動から保護します

Kolaviron protects against cognitive deficits and cortico-hippocampal perturbations associated with maternal deprivation in rats.

• Gabriel Olaiya Omotoso
• Nafisat Yetunde Mutholib
• Fatimah Adeola Abdulsalam
• Abdulkabir I Bature

抄録

生後間もない時期に母親から離れた子犬は、正常な成長と発育を妨げる可能性があり、その結果、人間の統合失調症の特徴に類似した異なる行動変化が生じる。本研究では、ビフラボノイドであるコラビロンの細胞保護作用を、母性剥奪したWistarラットの前頭前野と海馬で検討した。A群は通常の生理食塩水0.5ml、B群は産後21～35日目にコラビロン経口投与（200mg/kg/bw）、C群は産後9日目に24時間、D群は産後9日目に24時間、その後21～35日目にコラビロン経口投与（200mg/kg/bw）を行った。行動試験（オープンフィールド試験、モリス水試験、Ｙ迷路試験）は、実験後、生け贄に先立って実施した。ラットの一部はケタミンで麻酔し、0.1Mリン酸緩衝生理食塩水と4％パラホルムアルデヒドで灌流固定したが、他のラットは酵素研究のために頸椎脱臼で犠牲にした。海馬と前頭前野は、脳から切除し、組織組織学、組織化学、および酵素分析のために処理した。その結果、母性喪失ラットの前頭前野と海馬では、行動障害、酸化ストレス、変性変化、アストロサイトーシスが認められたが、コラビロンの介入により、これらの脳領域の神経行動、形態学、神経化学に有意な改善がみられた。我々は、コラビロンは、産後早期の期間に母親の分離から生じる栄養や環境の侮辱の神経学的な結果から脳を保護することができると結論づけた。

Prolonged separation of pups from their mother in early postnatal period can interfere with normal growth and development, resulting in different behavioral changes similar to features of schizophrenia in man. This study explored the cytoprotective action of kolaviron, a biflavonoid, on the prefrontal cortex and hippocampus of maternally deprived Wistar rats. Eight months old female rats were time-mated, and after delivery their pups were randomly assigned into four groups; group A received 0.5 ml of normal saline, group B received kolaviron orally (200 mg/kg/bw) on postnatal days (PND) 21-35, group C were maternally deprived on PND 9 for 24 hours, while group D were also maternally deprived on PND 9 for 24 hours, and then received kolaviron orally (200 mg/kg/bw) on PND 21-35. Behavioral studies (open field test, Morris water test, and Y-maze test) were conducted after the experiment prior to sacrifice. Some of the rats were anesthetized with ketamine and perfusion-fixed with 0.1 M phosphate buffered saline and 4% paraformaldehyde, while others were sacrificed by cervical dislocation for enzyme studies. The hippocampus and prefrontal cortex were excised from the brain and processed for tissue histology, histochemistry, and enzymatic analysis. Results revealed behavioral deficits, oxidative stress, degenerative changes, and astrocytosis in the prefrontal cortex and hippocampus of maternally deprived rats, but intervention with kolaviron caused significant improvement in neurobehavior, morphology, and neurochemistry in these brain areas. We concluded that kolaviron could protect the brain against neurological consequences of nutritional and environmental insults arising from maternal separation in early postnatal period.

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