Bixa orellana 抽出物と青色 LED を用いた抗菌性光線力学的治療による口臭の軽減-無作為化比較臨床試験

Antimicrobial photodynamic therapy with Bixa orellana extract and blue LED in the reduction of halitosis-A randomized, controlled clinical trial.

• Marcela Leticia Leal Gonçalves
• Ana Carolina Costa da Mota
• Alessandro Melo Deana
• Lisyanne Araújo de Souza Cavalcante
• Anna Carolina Ratto Tempestini Horliana
• Christiane Pavani
• Lara Jansiski Motta
• Kristianne Porta Santos Fernandes
• Raquel Agnelli Mesquita-Ferrari
• Daniela Fátima Teixeira da Silva
• Pamella de Barros Motta
• Renato Araújo Prates
• Sandra Kalil Bussadori

抄録

背景:

本研究では、Bixa orellana抽出物と青色発光ダイオード（LED）を用いた抗菌性光力学療法（aPDT）を行った場合の口臭の軽減効果を評価することを目的とした。

BACKGROUND: This study aimed to evaluate the reduction of halitosis when using antimicrobial photodynamic therapy (aPDT) with Bixa orellana extract and blue light-emitting diode (LED).

方法:

ガスクロマトグラフィーで硫化物（H2S）≧112ppbと診断されたUNINOVEの学生または社員44名を抽出した。患者を無作為にグループに分けた。グループ１（ｎ＝１５）：アナトーとＬＥＤを用いたａＰＤＴ；グループ２（ｎ＝１４）：舌掻き；グループ３（ｎ＝１５）：舌掻きとａＰＤＴ。aPDTについては、青紫色LED（Valo Cordless Ultradent® Products, Inc.舌の裏側に6点を、波長395〜480nmで20秒間、エネルギー9.6J、1点あたりの放射エネルギー6.37J/cmで照射した。治療前、治療直後、7日後を比較した。グループ内分析にはFriedman検定を、グループ間分析にはKruskal Wallis検定を用いた。

METHODS: Forty-four UNINOVE students or employees with a diagnosis of sulfide (H2S) ≥ 112 ppb in gas chromatography were selected. The patients were randomly divided in groups: Group 1 (n = 15): aPDT with annatto and LED; Group 2 (n = 14): tongue scraping; Group 3 (n = 15): tongue scraping and aPDT. For aPDT, a wasBixa orellana extract used in a concentration of 20 % w/v (Fórmula e Ação®, São Paulo, Brazil) on the tongue for 2 min, associated with a blue-violet LED (Valo Cordless Ultradent® Products, Inc., South Jordan, UT, USA) (395-480 nm). Six points were irradiated on the back of the tongue, at wavelength 395-480 nm for 20 s, energy of 9.6 J and radiant energy of 6.37 J/cm per point. The results were compared before, immediately after treatment and 7 days after. The Friedman test was used for the intragroup analysis and the Kruskal Wallis test for the intergroup analysis.

結果:

全ての群で、ベースラインと治療直後の値に差があった。グループ１とグループ３では、ベースラインと７日間の対照値との間に差はなかった。

RESULTS: In all groups, there was a difference between baseline and the value immediately after the treatment. In Groups 1 and 3, there was no difference between the baseline and the 7 days control.

結論:

即座に口臭の軽減が認められたが、7日後には軽減が維持されなかった。

CONCLUSION: There was an immediate reduction of halitosis, but the reduction was not maintained after 7days.

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