Viola tricolor Linn.の心臓保護および血圧降下剤としてのEx vivoおよびin vivoでの研究。電圧ゲーテッドCaイオンチャネルの阻害

Ex vivo and in vivo studies of Viola tricolor Linn. as potential cardio protective and hypotensive agent: Inhibition of voltage-gated Ca ion channels.

• Fatima Saqib
• Khizra Mujahid
• Muhammad Arif Aslam
• Alotaibi Modhi
• Marius Alexandru Moga
• Elena Bobescu
• Luigi Marceanu

抄録

は、伝統医学では心臓保護・降圧剤として使用されている。本研究の目的は、Viola tricolor L.の心臓保護効果と降圧効果をin vitro試験およびin vivo試験で評価することであった。Viola tricolor L.の粗抽出物(Vt.Cr)とその画分(水性と有機)をウサギの心房と大動脈で試験し、in vitroでは心臓抑制作用と血管弛緩作用を、in vivoでは正常血圧のケタミンジアゼパム麻酔ラットでは侵襲的な方法で血圧を調べた。急性心筋梗塞や左室肥大の発症にはイソプロテレノールを用い，Vt.Crの心保護効果を血行動態や病理組織学的に評価した．Vt.Crとその分画は、ペアになった心房の心拍数と収縮力を減少させ、リラックスした状態ではフェニルフリン(1µM)とK(80mM)が大動脈の収縮を刺激し、生体内での降圧作用に支えられた電圧依存性のL型カルシウムチャネル遮断を介している可能性がある。LVHでは、Vt.Crはアンジオテンシン変換酵素とレニンを低下させ、環状グアノシン一リン酸と一酸化窒素レベルを増加させ、高速液体クロマトグラフィーで検出されたガリン酸に起因する心筋細胞のサイズと線維化を減少させました。部分的な肯定的な結果は、血行動態学的にも組織学的にもAMIビオラトリコロールL.に見られました。

Viola tricolor Linn. is used as cardio-protective and anti-hypertensive agent in traditional medicine. Current study objective was to evaluate cardio-protective and hypotensive effects of Viola tricolor L. in vitro and in vivo studies. Viola tricolor L. crude extract (Vt.Cr) and its fractions (Aqueous and organic) were tested at rabbit atria and aorta coupled to Power Lab Data Acquisition System for cardio depressant and vasorelaxant effects in vitro whereas in vivo Blood Pressure was checked by invasive method in normotensive ketamine-diazepam anesthetized rats. Isoproterenol was employed for acute myocardial infarction (AMI) and left ventricular hypertrophy (LVH) development and cardioprotective effects of Vt.Cr were evaluated hemodynamically and histopathologically. Vt.Cr and its fractions decreased heart rate and contractile force in paired atria and relaxed Phenylephrine (1 µM) and K (80 mM) stimulated contractions in aorta possibly mediated through Voltage dependent L-type calcium channels blockage supported by in vivo hypotensive action. In LVH, Vt.Cr lowered Angiotensin Converting Enzymes and renin, increased cyclic Guanosine Monophosphate and nitric oxide levels, decreased cardiomyocytes size and fibrosis attributed to Gallic acid as detected by High Performance Liquid Chromatography. Partial positive results were seen hemodynamically and histologically in AMI Viola tricolor L. showed vasorelaxant, cardio-relaxant, hypotensive, and cardio protective effect validating traditional practice in cardiovascular disorders.

© 2020 Federation of American Societies for Experimental Biology.