アルギニンを添加した飼料を給与した金目鯛（Sparus aurata L.）の皮膚創傷治癒

Skin wound healing in gilthead seabream (Sparus aurata L.) fed diets supplemented with arginine.

• Zhichu Chen
• Diana Ceballos-Francisco
• Francisco A Guardiola
• Dong Huang
• M Ángeles Esteban

抄録

金目鯛の創傷治癒過程に及ぼすアルギニンの食事投与について検討した。市販の飼料（コントロール飼料（CON）、アルギニン無添加）と1%アルギニン（ARG1）または2%アルギニン（ARG2）を添加したCON飼料を30日間与えた魚（n=8）を2匹複製した。その後、半分の魚をサンプリングしながら、残りの半分は傷をつけ、さらに1週間同じ飼料を与え続けた。画像解析の結果、ARG1食を与えた魚では、CON群と比較して創傷閉鎖率が有意に改善された。創傷治癒7日後、血清中のアミノトランスフェラーゼおよびクレアチンキナーゼレベル、皮膚粘液中のプロテアーゼおよびペルオキシダーゼ活性は低下し、皮膚粘液中の免疫グロブリンMレベルは創傷後のARG1投与群および創傷前のCON投与群で上昇していた。ARG1食はCON食と比較して、mpo、il-8、tnf-αの遺伝子発現を著しく低下させ、創傷後の皮膚組織におけるtgf-β1、igf-1、pcna、krt2、mmp9、fn1α、colIα、および抗酸化酵素catの遺伝子発現を亢進させた。さらに、ARG1群およびCON群と比較して、ARG2食の創傷治癒に対する負の効果が示された。結論として、1％のアルギニン補給は皮膚創傷部位の炎症反応を緩和し、皮膚創傷部位の再上皮化とECM生合成を促進することで、皮膚創傷治癒を促進し、全身性の炎症反応を防ぐことができた。

Dietary administration of arginine on the wound healing process of gilthead seabream was studied. Two replicates of fish (n = 8) were fed with either a commercial diet [control diet (CON), no arginine added] and the CON diet supplemented with 1% arginine (ARG1) or with 2% arginine (ARG2) for 30 days. Afterward, half of the fish were sampled while the other half were injured and continued to be fed the same diet for an extra week. Results by image analysis showed that the wound closure rate was significantly improved in fish that were fed the ARG1 diet, compared with those in the CON group. After seven days of wound healing, the aminotransferase and creatine kinase levels in the serum and the protease and peroxidase activities in the skin mucus were down-regulated, while the immunoglobulin M level in the skin mucus was up-regulated in the ARG1 group after wounding and in the CON group before wounding. Compared with the CON diet, the ARG1 diet remarkedly depressed the gene expression of mpo, il-8, and tnf-α, and enhanced the gene expression of tgf-β1, igf-1, pcna, krt2, mmp9, fn1α, and colIα and the antioxidant enzyme cat in the skin tissues after wounding. Furthermore, compared with both the ARG1 and the CON groups, negative effects of the ARG2 diet on wound healing were demonstrated. In conclusion, a 1% arginine supplementation facilitates skin wound healing and prevents a systemic inflammation reaction by alleviating the inflammatory response and enhancing the re-epithelialization and ECM biosynthesis in skin wound sites.

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