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ヒマシ抽出物の静脈内注射による致死的中毒の一例
Fatal intoxication by intravenous injection of castor bean (Ricinus communis L.) extract-a case study.
PMID: 32548760 DOI: 10.1007/s00414-020-02340-x.
抄録
25歳の男性が自家製ヒマシエキス水溶液を静脈内注射して自殺した症例を報告した。患者は入院して対症療法を受け、酩酊状態から4日目に本人の希望で退院した。翌日に病状が悪化し、治療を受けたが中毒後6日目に死亡した。症例歴、剖検、剖検前および剖検後の採取物の毒物学的調査について報告した。剖検前に患者から採取した血液と尿、剖検時に採取したその他の生体物質（上肢および下肢の血液、左右心室の血液、心嚢液、硝子体液、肝臓、腎臓、脾臓）を剖検後に採取した。リシン/キャスターシード中毒のバイオマーカーとしてのリシニンの同定と定量のための液液液抽出法と高速液体クロマトグラフィー・タンデム質量分析法を開発し、検証した。この方法は、採取した死前及び死後の生物学的試料の分析に適用した。まず、剖検後の臓器(肝臓,腎臓,脾臓)におけるリシニンの含有量を報告した。得られた結果から、死後の体内ではリシニンがほぼ均一に分布していることが明らかになった（濃度は1ngmL程度）。また、ヒマシの種子から抽出した抽出物や患者の尿の分析にもGC-MS法を適用し、臨床・法医学的なリシニンの同定の可能性を示した。
A case report of a 25-year-old man who committed suicide by intravenous injection himself of an aqueous home-made castor bean extract is presented. The patient was hospitalized and treated symptomatically and was released at its own request fourth day after intoxication. The next day, the patient's condition deteriorated, and he died 6 days after intoxication even though he was given medical care. Case history, autopsy, and toxicological investigation of ante- and post-mortem collected materials are described. Blood and urine collected from the patient ante-mortem and other several biological materials (namely blood from the upper and lower limb, blood from the right and left ventricle, pericardial fluid, vitreous humour, liver, kidney, and spleen) were collected post-mortem during autopsy. Liquid-liquid extraction procedure followed by high-performance liquid chromatography tandem mass spectrometry analysis for identification and determination of ricinine as a biomarker of ricin/castor seed intoxication was developed and validated. The method was applied on analysis of collected ante- and post-mortem biological materials. The post-mortem contents of ricinine in organs (namely the liver, kidney, and spleen) are firstly reported. The obtained results indicated approximately uniform distribution of ricinine (concentration level about 1 ng mL) in the body after death. In addition, the GC-MS method was also applied for the analysis of extract of castor seed and the patient's urine, to demonstrate alternative possibility for identification of ricinine for clinical and forensic purposes.