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ペクチン系銅ナノ粒子のグリーンで効率的な生合成とその抗菌活性
Green and efficient biosynthesis of pectin-based copper nanoparticles and their antimicrobial activities.
PMID: 32572568 DOI: 10.1007/s00449-020-02390-w.
抄録
ここでは、ペクチンを安定化剤として、アスコルビン酸を還元剤として用いることで、マイクロ波照射条件での銅ナノ粒子（CuNPs）のグリーン生合成法を報告した。最適な条件で、マイクロ波照射時間0分、3分でCuNPs1、2を合成した。透過型電子顕微鏡及び走査型電子顕微鏡(SEM)試験の結果、CuNPs1及び2の平均粒子径はそれぞれ61.9±19.4及び40.9±13.6nmの不規則な多角形粒子であることがわかった。ゼータ電位はそれぞれ-45.2mV及び-48.7mVであった。X線回折,フーリエ変換赤外分光法(FTIR),熱重量分析法(TGA)及びX線光電子分光法を用いてCuNPsの特性を評価した。さらに、阻害領域試験の結果、CuNPs2はCuNPs1よりも大腸菌、黄色ブドウ球菌、アスペルギルス・ジャポニカスに対して高い抗菌活性を示した。また、ブロス培地中での細菌増殖速度を調べたところ、CuNPs2はCuNPs1よりも低い最小殺菌濃度を示した。
Herein, we reported a green biosynthesis method of copper nanoparticles (CuNPs) at microwave irradiation condition by using pectin as a stabilizer and ascorbic acid as a reducing agent. Under the optimum conditions, CuNPs1 and 2 were synthesized under microwave times 0 and 3 min, respectively. Transmission electron microscope and scanning electron microscope (SEM) tests showed that CuNPs1 and 2 had irregular polygon particles with average diameters of 61.9 ± 19.4 and 40.9 ± 13.6 nm, respectively. Zeta potentials of CuNPs1 and 2 were -45.2 and -48.7 mV, respectively. X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), and X-ray photoelectron spectroscopy techniques were used to characterize the properties of CuNPs. Furthermore, inhibition zone tests showed that CuNPs2 exhibited higher antimicrobial activities against Escherichia coli, Staphylococcus aureus, and Aspergillus japonicus than CuNPs1. The antibacterial activities were also studied by the bacterial growth kinetics in broth media, and CuNPs2 exhibited lower minimum bactericidal concentrations than CuNPs1.