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ホウ酸を添加した魚類飼料を摂取したニジマス(Onchorhyncus mykiss)の組織病理学的変化
Histopathology changes in the rainbow trout (Onchorhyncus mykiss) consuming boric acid supplemented fish fodder.
PMID: 32593084 DOI: 10.1016/j.jtemb.2020.126581.
抄録
研究の目的:
ホウ素(B)は微量微量栄養素として自然界や生物界に存在していますが、その濃度が高いと生物に有害な影響を与える可能性があり、また毒性もあることが知られています。本研究の目的は、飼料に添加したホウ素酸(BA)がニジマス(Oncorhynchus mykiss)に及ぼす影響を病理組織学的に検討し、対照飼料と比較した(飼料中のホウ素の0.01%、0.05%、0.10%、0.20%)。
OBJECTIVE: Even though boron (B), as a trace micronutrient, occurs in natural waters and organisms, its high concentration could cause harmful and even toxic for organisms. The aim of present study was to investigate the effects of boric acid (BA) added to feed (0.01%, 0.05%, 0.10%, and 0.20% of B in feed) on Rainbow Trout (Oncorhynchus mykiss) by histopathological methods and compared to the control feed as without B.
方法論:
摂食終了時に、各群から9匹のニジマスの生体重を秤量した後、組織片を肝臓、鰓、腎臓、骨格筋、脾臓、脳から採取し、全身の壊死が行われた魚の組織片を10%緩衝ホルマリン溶液で固定した。
METHODOLOGY: At the end of the feeding, after weighing the live weight of 9 rainbow trout from each group, tissue fragments were taken from the liver, gill, kidney, skeletal muscle, spleen and brains of fish, which systemic necropsies was done, and were fixed in 10 % buffered formalin solution.
結果:
病理組織学的検査の結果、肝臓、鰓、腎臓、骨格筋、脾臓、脳に変性と壊死の変化が認められた。肝実質の水和性および液胞性変性、エラのラメラ浮腫、腎臓のヒアリン蓄積、筋肉の変性、脾臓の壊死、脳の充血が対照群を除く全ての群で観察された。魚類に対するホウ酸の破壊は、ホウ素の添加量に依存して増加することが観察され、0.20%ホウ素添加飼料を与えた群で最も効果があった。
RESULTS: Histopathological examinations revealed degenerative and necrotic changes in the liver, gill, kidney, skeletal muscle, spleen and brain. Hydropic and vacuolar degenerations in liver parenchyma, lamellar edema in the gills, hyaline accumulation in the kidneys, degenerations in the muscles, necrosis in the spleen and hyperemia in the brain were observed in all groups except control group. It was observed that the destruction of boric acid on fish increased depending on the amount of boron supplemented to the feed, and the most effect was in the group fed with 0.20 % boron supplemented feed.
結論:
ホウ酸(BA)の病理組織学的所見では、全ての臓器に病理学的変化を引き起こすことはさておき、最も重篤な病変は肝臓に認められた。
CONCLUSIONS: The findings aside from causing pathological changes in all organs in terms of histopathological findings of Boric Acid (BA), the most severe lesions were observed in the liver.
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