乳癌患者における循環CD28陰性サプレッサーT細胞とメモリーB細胞の予後的意義

Prognostic Significance of Circulating CD28 Negative Suppressor T Cells and Memory B Cells in Patients with Breast Cancer.

• Asmaa M Zahran
• Asmaa S Shaltout
• Hussein Fakhry
• Salah M Khallaf
• Ola N Abdel Fattah
• Doaa F Temerik
• Amal Rayan

抄録

背景:

異なるリンパ球サブセットを日常的に評価し、定量化することで、乳がん（BC）における臨床的に意味のある予後情報を提供できることが示唆された。

BACKGROUND: It has been suggested that routine assessment and quantification of different lymphocyte subsets can provide clinically meaningful prognostic information in breast cancer (BC).

目的:

BC患者における末梢血リンパ球サブセットと病理学的パラメータと治療反応との関係を明らかにする。

OBJECTIVE: To determine the relationship between peripheral blood lymphocyte subsets and pathological parameters and response to therapy in patients with BC.

方法:

手術可能な乳がん患者30名と健常者20名を対象とした。末梢血中のリンパ球サブセットの検出には、フルオロクローム標識モノクローナル抗体を使用し、フローサイトメトリーで細胞を分析した。患者は化学療法、放射線療法、ホルモン療法で治療され、再発および無再発生存期間（RFS）を決定するために追跡調査された。

METHODS: Thirty patients with operable breast cancer treated surgically with either modified radical mastectomy or breast conservative surgery, and 20 healthy controls were included. For detection of lymphocyte subsets in peripheral blood; Fluorochrome-labeled monoclonal antibodies were used andcells were analyzed by flow cytometry. Patients were treated with chemotherapy, radiotherapy and hormonal treatment, and followed up to determine relapse and recurrence-free survival (RFS).

結果:

BC患者と対照群の間では、B、T、NK、NKT、CD28-T細胞の頻度に有意差が認められた。さらに、異なる病理学的サブタイプのBC患者間ではCD8+CD28-T細胞の割合に有意差が認められ、CD8+CD28-T細胞とメモリーB細胞の頻度とRFSとの間には負の相関が観察されました。また、腫瘍グレードの異なる患者では、ナイーブB細胞の頻度に有意な差が認められ、B細胞とNKT細胞の頻度の間には負の相関が認められた。

RESULTS: Significant differences were found in the frequencies of B, T, NK, NKT, and CD28‒T cells between patients with BC and controls. Moreover, a significant difference was found in the percentage of CD8+CD28‒ T cells between patients with different pathologic subtypes of BC and negative correlations were observed between the frequency of CD8+CD28‒T cells and memory B cells, and RFS. Also, a significant difference in the frequency of naïve B cells was found in patients with different tumor grades and a negative correlation was found between the frequencies of B cells and NKT cells.

結論:

NK、NKT、リンパ球、CD28-T細胞は健常対照者とBC患者で有意に異なっていた。また、メモリーB細胞は治療効果が高いのに対し、CD28-T細胞はRFSが短かった。

CONCLUSION: NK, NKT, lymphocytes, and CD28‒ T cells significantly differed between healthy controls and BC patients. Also, memory B cells were associated with good response to treatment while CD28‒ T cells were associated with shorter RFS.