OprF/OprI/PcrV組換えキメラ蛋白質の抗炎症作用の検討

Protective Efficacy of the OprF/OprI/PcrV Recombinant Chimeric Protein Against in the Burned BALB/c Mouse Model.

• Mohammad Hadi Fakoor
• Seyed Latif Mousavi Gargari
• Parviz Owlia
• Azar Sabokbar

抄録

背景:

感染は熱傷患者の主な死因である。そこで、本研究では、OprF-OprI-PcrVを内包したキメラワクチンを設計し、OprF-OprI-PcrVをマウスに発現させた。熱傷を負ったマウスを用いて、OprI、OprF、PcrVの免疫原性を調べた。

Background: infection is the major cause of death in burn patients. Thus, in this study, a chimeric vaccine harboring the OprF-OprI-PcrV was designed and expressed in . The immunogenicity of the recombinant chimer, OprI, OprF, and PcrV was studied in a burned mouse model.

方法論:

提案されたキメラ、OprF、OprI、PcrVを含む組換え蛋白質をマウスの火傷モデルで発現させた。 精製された組換え蛋白質をマウスに免疫し、免疫したマウスから得られた血清中の抗体価を推定した。免疫したマウスと対照マウスに、本菌株（PAO1, PAK, R）の2, 5, 10xLDを負荷し、皮膚、肝臓、脾臓、腎臓の微生物数を測定した。

Methodology: Recombinant proteins including the proposed chimer, OprF, OprI, and PcrV were expressed in the . Mice were immunized with the purified recombinant proteins, and the antibody titre was estimated in the sera obtained from immunized mice. Immunized and control mice were challenged with 2, 5, and 10xLD of the strains (PAO1, PAK, and R), and microbial counts were measured in the skin, liver, spleen, and kidney of the studied mice.

結果:

その結果、対照群に比べて実験群では10μgのキメラ蛋白質を注射することで抗体価（総IgG）が有意に上昇することが示された。また、2回目のブースター投与後235日目まで抗体生存力価が高かった。10xLDに感染したマウスの生存率は、OprF、OprI、PcrVタンパク質のいずれかを単独で免疫したマウスと比較して、特に内臓（腎臓、脾臓、肝臓）の細菌数が有意に増加し、細菌数が減少した。

Results: Results showed that the antibody titre (total IgG) was significantly increased by injection of 10 μg of chimeric protein in the experimental groups compared to the control groups. The antibody survival titre was high until 235 days after administration of the second booster. The survival rate of the mice infected with 10xLD was significantly increased and the number of bacteria was reduced, especially in the internal organs (kidney, spleen, and liver) compared to the mice immunized with any of the OprF, OprI, and PcrV proteins alone.

結論:

本研究の結果、キメラ蛋白質は感染症の制御のための有望なワクチン候補であることが明らかになった。

Conclusion: The findings of our study revealed that the chimeric protein is a promising vaccine candidate for control of the infection.

© 2020 Fakoor et al.