日本語AIでPubMedを検索
電子移動プローブにCuSマイクロフローを、信号増幅にAu@COFを用いた分子インプリント電気化学センサーを用いたスルファチアゾールの超高感度定量
Ultrasensitive determination of sulfathiazole using a molecularly imprinted electrochemical sensor with CuS microflowers as an electron transfer probe and Au@COF for signal amplification.
PMID: 32615382 DOI: 10.1016/j.foodchem.2020.127376.
抄録
本研究では、硫化銅(CuS)を新規なシグナルプローブとして採用した分子インプリントセンサーを開発し、スルファチアゾール(STZ)の超高感度かつ選択的な定量に成功した。多量のCuを含むCuSの電子移動の過程で生じるCuの還元シグナルが捕捉されやすく、高い電気化学的シグナルが得られます。さらに、電極表面に導電性に優れた金ナノ粒子＠共有有機骨格を導入することで、信号の増幅とCuSの電子移動過程の促進を図った。最適化された試験条件の下で、提案したセンサーは、非常に広い濃度範囲（1.0×10～1.0×10 mol L）でSTZに対して直線的なDPV応答を示し、検出限界は4.3×10 mol Lであった。さらに，提案したセンサーを用いて鶏肝臓および豚肝臓中のSTZ濃度を測定したところ，高速液体クロマトグラフィーで測定した濃度とほぼ同じ定量結果が得られた。
In this work, a molecularly imprinted sensor employing copper sulfide (CuS) as a novel signal probe was successfully developed for ultrasensitive and selective determination of sulfathiazole (STZ). The reduction signals of Cu produced in the process of electron transfer of CuS containing large amounts of Cu are easy to be captured, which provide high electrochemical signals. Moreover, gold nanoparticles@covalent organic framework with excellent conductivity was introduced on the electrode surface for signal amplification and facilitating electron transfer processes of CuS. Under optimized testing conditions, the proposed sensor offered a linear DPV response to STZ over a very wide concentration range (1.0 × 10 to 1.0 × 10 mol L), with a limit of detection of 4.3 × 10 mol L. Fodder and mutton samples spiked with STZ were analyzed using this sensor, and the satisfactory recoveries ranging from 83.0% to 107.2% were obtained. In addition, the proposed sensor was used to determine the concentration of STZ in chicken liver and pork liver, with quantification results being near identical to those determined by high-performance liquid chromatography.
Copyright © 2020 Elsevier Ltd. All rights reserved.