5-アミノレブリン酸光力学療法のバイオフィルムへの影響

[Effect of 5-aminolevulinic Acid Photodynamics Therapy on Biofilm of ].

• Yuzhen Liu
• Rong Zeng
• Zhimin Duan
• Haoxiang Xu
• Qiuju Wu
• Qing Chen
• Tong Lin
• Min Li

抄録

5-アミノレブリン酸光力学療法(ALA-PDT)のバイオフィルムに対する効果を調べるために、細胞スライド上にバイオフィルムを構築し、ALA-PDTで処理した。バイオフィルムは、異なる光量に応じて、ALA-PDT群[ALA-PDT1群(50J/cm),ALA-PDT2群(100J/cm),ALA-PDT3群(200J/cm)]、ALAのみ群(ALA群)、光のみ群(LED群)、ネガティブコントロール群(ALA-PDT群)の6つの群に分けた。バイオフィルムの構造と死菌/生菌比をレーザー共焦点顕微鏡（CLSM）を用いて観察し、XTTアッセイを用いてバイオフィルムの生存率を測定した。 その結果、ALA-PDT群、ALA群、LED群、ALA-PDT1群、ALA-PDT2群、ALA-PDT3群の死菌/生菌比は0.0であり、ALA-PDT群、ALA群、LED群、ALA-PDT1群、ALA-PDT2群、ALA-PDT3群の死菌/生菌比は0.0であった。350±0.033、0.305±0.046、0.330±0.032、1.525±0.439、2.293±0.148、3.092±0.189であった。000）はALA-PDT群と有意差はなかったが、ALA-PDT群の死菌/生菌比はALA-PDT1群（=-0.162、&lt;0.001）に比べて有意に低い値を示した。162, <0.001），ALA-PDT2群（=-0.254, <0.001），ALA-PDT3群（=-0.352, <0.001）と比較して，ALA-PDT群の死菌/生菌率は有意に低く，XTTアッセイの値は0.462±0.028，0.465±0.044，0.465±0.044であった。ALA-PDT群、ALA群、LED群、ALA-PDT1群、ALA-PDT2群、ALA-PDT3群では、それぞれ437±0.047、0.301±0.040、0.207±0.001、0.110±0.007であった。ALA(=-0.044, =1.000)、LED群(=-0.020, =1.000)のXTT測定値は、ALA-PDT群と大きな差はありませんでしたが、ALA-PDT群ではALA-PDT1群に比べて有意に高い値を示しました(=1.175, &lt;0.001)。175、<0.001）、ALA-PDT2群（=1.942、<0.001）、ALA-PDT3群（=0.352、=2.742、<0.001）と比較して、ALA-PDT群の方が有意に高かった。 ALA-PDTはバイオフィルムの構造を破壊し、バイオフィルムの生存率を抑制する効果があります。

To investigate the effect of 5-aminolevulinic acid photodynamic therapy (ALA-PDT) on () biofilm. biofilms were constructed on a cell slide and treated with ALA-PDT.According to different light doses,the biofilms were divided into six groups:ALA-PDT group [ALA-PDT1 (50 J/cm),ALA-PDT2 group (100 J/cm),ALA-PDT3 group (200 J/cm)],ALA-only group (ALA group),light-only group (LED),and a negative control group (ALA-PDT-group).The biofilm structure and the ratio of the dead bacteria/live bacteria were observed using a laser confocal microscope (CLSM).Biofilm viability was measured using the XTT assay. CLSM showed that the biofilm structures of ALA group and LED group were not significantly different from that of ALA-PDT-group,whereas the biofilm structure was more seriously damaged in ALA-PDT1 group,ALA-PDT2 group,and ALA-PDT3 group than in the ALA-PDT-group.The ratios of the dead/live bacteria in ALA-PDT-group,ALA group,LED group,ALA-PDT1 group,ALA-PDT2 group,and ALA-PDT3 group were 0.350±0.033, 0.305±0.046, 0.330±0.032, 1.525±0.439, 2.293±0.148 and 3.092±0.189,respectively.ALA group(=0.003, =1.000)and LED group(=-0.025, =1.000)did not significantly differ from the ALA-PDT-group.However,the ratio of dead/live bacteria in ALA-PDT-group was significantly lower than those in ALA-PDT1 group (=-0.162, <0.001),ALA-PDT2 group (=-0.254, <0.001),and ALA-PDT3 group (=-0.352, <0.001).The values of the XTT assay were were 0.462±0.028,0.465±0.044,0.437±0.047,0.301±0.040,0.207±0.001,and 0.110±0.007,respectively,in ALA-PDT-group,ALA group,LED group,ALA-PDT1 group,ALA-PDT2 group,and ALA-PDT3 group.Although the values of XTT assay in ALA(=-0.044, =1.000)and LED groups (=-0.020, =1.000)did not significantly differ from that in ALA-PDT-group,it was significantly higher in ALA-PDT-group than in ALA-PDT1 group (=1.175, <0.001),ALA-PDT2 group (=1.942, <0.001),and ALA-PDT3 group (=-0.352, =2.742, <0.001). ALA-PDT has an inhibitory effect on biofilm.ALA-PDT destroys biofilm structure and inhibits biofilm viability.