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錬金術前後の桂林寺の化学組成の違いに関する質量分析と多変量統計解析による研究
[Study on difference of chemical compositions of Guilingji before and after alchemy based on mass spectrometry and multivariate statistical analysis].
PMID: 32627462 DOI: 10.19540/j.cnki.cjcmm.20200328.311.
抄録
UPLC-Q-Orbitrap MS/MS及びICP-MSと多変量統計解析を組み合わせて、錬金術前後の桂林寺の化学組成の違いを調べた。錬金術後のGLJ中には、バイオレットケトン、カルコン、アミド、脂肪酸が含まれており、錬金術後に含有量が増加したほか、フラボン、イソフラボン、ジヒドロフラボン、フラボノイド配糖体、クマリンが含まれており、錬金術後に含有量が減少した。錬金前と錬金後のGLJを区別するためのマーカーとして、B, Si, Mg, K, Cr, Niの6つの無機元素をろ過した結果、Mg, K, Cr, Niの含有量は増加し、錬金後はBとSiの含有量が減少した。本研究では、錬金術前後のGLJの化学組成の変化を予備的に検討するとともに、錬金術がGLJの物理的・化学的性質や冷熱性に及ぼす影響を検討し、錬金術プロセスの科学的意味合いをより明確にするための基礎を築いた。
UPLC-Q-Orbitrap MS/MS and ICP-MS coupled with multivariate statistical analysis was employed to explore the differences in chemical compositions of Guilingji(GLJ) before and after alchemy.The changes in organic chemical compositions and inorganic elements were observed and 39 differential organic compositions were found in GLJ after alchemy, 24 compounds of which were identified. The differential compositions of GLJ included violet ketones, chalcones, amides, and fatty acids whose contents were increased after alchemy, as well as flavones, isoflavones, dihydroflavones, flavonoid glycosides, and coumarins whose content were decreased after alchemy. This study showed 6 inorganic elements filtered out as markers for distinguishing GLJ before and after alchemy, including B, Si, Mg, K, Cr, and Ni.The contents of Mg, K, Cr and Ni were increased while the contents of B and Si were decreased after alchemy.The difference of the contents after alchemy changed the cold and hot properties of the compound, showing the decrease of dryness, and the hot property was changed to warm and neutral properties; in addition, the membrane permeability and absorption of the compound compositions were improved. In this study, we preliminarily investigated the changes of chemical compositions in GLJ before and after alchemy as well as the effects of alchemy on physical and chemical properties and cold-heat nature of GLJ, laying a foundation for further clarifying the scientific connotation of alchemy process.