悪性胸水を有する肺癌患者におけるB7-H3およびmiR-145の発現量と臨床的意義について検討した

The expression and clinical significance of B7-H3 and miR-145 in lung cancer patients with malignant pleural effusion.

• L Huang

抄録

目的:

悪性胸水を有する肺癌患者におけるコスティマイモロジー分子B7-H3とmicroRNA-145（miR-145）の発現と臨床的意義を検討すること。

OBJECTIVE: To investigate the expression and clinical significance of costimulatory molecule B7-H3 and microRNA-145 (miR-145) in lung cancer patients with malignant pleural effusion.

患者と方法:

2017年3月から2018年9月までに当院に治療のために入院した肺がん患者を対象に、合計100例を抽出した。悪性胸水と診断された49例を調査群に、良性胸水と診断された51例を対照群に含めた。両群の胸水中のB7-H3の含有量を酵素免疫吸着法（ELISA）で検出し、両群の胸水中のmiR-145の発現をリアルタイム定量PCR（qRT-PCR）で解析した。B7-H3とmiR-145の発現と臨床病理学的特徴との関係を解析した。肺癌におけるB7-H3とmiR-145の診断値を解析した。

PATIENTS AND METHODS: A total of 100 cases of patients with lung cancer who admitted to our hospital for treatment from March 2017 to September 2018 were selected. Forty-nine cases of patients diagnosed with malignant pleural effusion were included in the study group, and 51 cases with benign pleural effusion in the control group. The content of B7-H3 in pleural effusion of the two groups was detected by enzyme-linked immunosorbent assay (ELISA), and the expression of miR-145 in pleural effusion of the two groups was analyzed by Real-time quantitative PCR (qRT-PCR). The relationships between the expressions of B7-H3 and miR-145 and the clinicopathological characteristics were analyzed. The diagnostic value of B7-H3 and miR-145 in lung cancer was analyzed.

結果:

試験群のB7-H3の発現レベルは対照群に比べて有意に高く（p＜0.050），miR-145の発現レベルは対照群に比べて有意に低かった（p＜0.050）．B7-H3およびmiR-145の発現量は、リンパ節転移、肺癌の分化度、TNM病期と相関していた（p<0.001）。肺癌の単発診断におけるmiR-145の感度および特異度はそれぞれ64.71％および79.59％であった。B7-H3の肺癌の単発診断における感度はそれぞれ80.39％、61.22％であった。

RESULTS: The expression level of B7-H3 in the study group was significantly higher than that in the control group (p<0.050), while the expression level of miR-145 was significantly lower than that in the control group (p<0.050). The expression levels of B7-H3 and miR-145 in the study group were correlated with lymph node metastasis, differentiation degree of lung cancer and TNM stage (p<0.001). The sensitivity and specificity of miR-145 in single diagnosis of lung cancer were 64.71% and 79.59%, respectively. The sensitivity and specificity of B7-H3 in single diagnosis of lung cancer were 80.39% and 61.22%, respectively.

結論:

B7-H3とmiR-145は肺がんで異常に発現しており、肺がんのリンパ系転移、分化度、TNM病期と密接に関連している。将来的には、肺がんの悪性胸水の診断マーカーになる可能性があります。

CONCLUSIONS: B7-H3 and miR-145 are abnormally expressed in lung cancer, and are closely related to the lymphatic metastasis, differentiation degree and TNM stage of lung cancer. They may be potential markers for the diagnosis of malignant pleural effusion in lung cancer in the future.