PRドメイン含有タンパク質12（prdm12）は、中枢神経系の細胞分化の際に転写因子zic1の下流標的となる

PR-domain containing protein 12 (prdm12) is a downstream target of transcription factor zic1 during cellular differentiation in central nervous system.

• Md Mahfujur Rahman
• In-Shik Kim
• Dongchoon Ahn
• Hyun-Jin Tae
• Byung-Yong Park

抄録

転写因子 zic1 は、神経プレートのパターニング、神経堤の形成、小脳の発生に重要な役割を果たしており、その主な機能は神経細胞の分化にある。prdm12は中脳背側、三叉神経節、脊髄の運動ニューロンに特異的に発現しており、prdm12のノックダウンは神経管の腹側化をもたらす。zic1をノックダウンすると中脳、運動ニューロン、三叉神経節でprdm12の発現が減少し、zic1を過剰に発現させると中脳でprdm12の発現が拡大する。我々は、prdm12がzic1の下流に位置し、ゼノパスの一部の神経節とともに脳細胞の分化を制御する遺伝子制御ネットワークの新たな担い手であることを提案する。

Transcription factor, zic1 is an important regulator of neural plate patterning, formation of neural crest and cerebellar development, where its main function is neuronal cell differentiation. Among the genes identified, PR domain-containing 12 (prdm12) is a member of the prdm family and is expressed in the placode domain in the neurula stage. prdm12 is distinctly expressed in the dorsal part of the midbrain, trigeminal ganglion and the motor neuron in the spinal cord. prdm12 knockdown results ventralization of neural tube. zic1 knockdown results in a reduction of prdm12 expression in the midbrain, motor neuron and trigeminal ganglion, and overexpression of zic1 results in an expansion of prdm12 expression in the midbrain. zic1 activated wnt signaling is also a regulator of prdm12 expression in the midbrain. We propose that prdm12 is downstream of zic1 and a novel player in the gene regulatory network controlling brain cell differentiation, along with some ganglions in Xenopus.

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