イネ蛋白質からのACE阻害性ペプチドの調製に対するマルチモードS型超音波前処理の効果

Effects of multi-mode S-type ultrasound pretreatment on the preparation of ACE inhibitory peptide from rice protein.

• Xue Yang
• Lingling Wang
• Fengying Zhang
• Haile Ma

抄録

胃腸模擬消化後のタンパク質加水分解物のACE阻害活性を指標に、マルチモードS型超音波を用いた超音波作用モードがイネ蛋白質（RP）からの生理活性ペプチドの調製に及ぼす影響を検討した。また、タンパク質および酵素分解物の構造特性についても検討した。その結果、すべての超音波処理モードでACE阻害活性が有意に増加し（p<0.05）、20/40kHz二周波超音波が最も有意な影響を示し、対照と超音波洗浄機に比べてそれぞれ38.28％、27.47％の増加が認められた。20/40kHz二周波超音波で前処理した後、RPの可溶性および疎水性タンパク質含量、遊離スルフヒドリル含量、表面疎水性が増加した。また、消化管模擬消化後のタンパク質加水分解物のペプチド含量と疎水性アミノ酸含量は、より高い値を示した（p<0.05）。結論として、マルチモードS型超音波前処理はRPからACE阻害ペプチドを調製する上で有効な方法であることがわかった。

The effects of ultrasound working modes using multi-mode S-type ultrasound on the preparation of bioactive peptide from rice protein (RP) were studied with the ACE inhibitory activity of protein-hydrolysate after gastrointestinal simulated digestion as the index. The structure characterizations of protein and enzymolysis products were also studied. Results showed that all the ultrasound working modes pretreatment increased the ACE inhibitory activity significantly (p < 0.05) and 20/40 kHz dual-frequency ultrasound showed the most significant impact, which increased by 38.28% and 27.47% compared to control and ultrasound cleaning machine, respectively. After pretreated by 20/40 kHz dual-frequency ultrasound, the soluble and hydrophobic protein contents, free sulfhydryl content and surface hydrophobicity of RP increased. And the peptide content and hydrophobic amino acid content of protein-hydrolysate after gastrointestinal simulated digestion showed higher value (p < 0.05). In conclusion, the multi-mode S-type ultrasound pretreatment is an effective way in preparation of ACE inhibitory peptide from RP.

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