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エンゼルフィッシュ(Pterophyllum scalare)およびオスカーフィッシュ(Astronotus ocellatus)由来のCryptosporidium bollandi n. sp.(Apicomplexa: Cryptosporidiiae)
Cryptosporidium bollandi n. sp. (Apicomplexa: Cryptosporidiiae) from angelfish (Pterophyllum scalare) and Oscar fish (Astronotus ocellatus).
PMID: 32659234 DOI: 10.1016/j.exppara.2020.107956.
抄録
形態学的,生物学的,分子学的特徴から,Cryptosporidium bollandi n. sp.の種名はCryptosporidium piscine genotype 2として提案された。18S rRNA(18S)配列の系統解析の結果、C. bollandi n. sp.はpiscine遺伝子型4に最も近縁であり(遺伝的距離5.1%)、Cryptosporidium molnari、Cryptosporidium huwi、Cryptosporidium scophthtalmiとの遺伝的距離はそれぞれ10.0%、12.2%、25.2%であった。アクチン遺伝子座では、C. bollandi n. sp.が再び最も近縁であり(遺伝的距離6.8%)、他のCryptosporidium属(Cryptosporidium felis)から15.5%(C. molnari)、18.4%(C. huwi)、22.9%(C. scophththalmi)、最大27.5%の遺伝的距離を示した。18Sおよびアクチン配列を連結した系統解析の結果、C. bollandi n. sp.は他のすべてのCryptosporidium spp.から12.9%(C. molnari)から21.1%(C. canis)の遺伝的距離を示した。 遺伝学的データおよび以前の組織学的解析から、C. bollandi n. sp.は別種としての妥当性が明確に支持されている。
The species name Cryptosporidium bollandi n. sp. is proposed for Cryptosporidium piscine genotype 2 based on morphological, biological and molecular characterisation. Phylogenetic analyses of 18S rRNA (18S) sequences revealed that C. bollandi n. sp. was most closely related to piscine genotype 4 (5.1% genetic distance) and exhibited genetic distances of 10.0%, 12.2% and 25.2% from Cryptosporidium molnari, Cryptosporidium huwi and Cryptosporidium scophthtalmi, respectively. At the actin locus, C. bollandi n. sp. was again most closely related to piscine genotype 4 (6.8% genetic distance) and exhibited 15.5% (C. molnari), 18.4% (C. huwi), 22.9% (C. scophthalmi) and up to 27.5% genetic distance from other Cryptosporidium spp. (Cryptosporidium felis). Phylogenetic analysis of concatenated 18S and actin sequences showed that C. bollandi n. sp. exhibited 12.9% (C. molnari) to 21.1% (C. canis) genetic distance from all other Cryptosporidium spp. Genetic data as well as previous histological analysis clearly supports the validity of C. bollandi n. sp. as a separate species.
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