カテコラミンとグルココルチコイドが .spp.の成長と病原性に及ぼす影響の検討

An Investigation of the Effect of Catecholamines and Glucocorticoids on the Growth and Pathogenicity of .

• Brendha Truccollo
• Paul Whyte
• Declan J Bolton

抄録

感染症の結果は軽度の腸炎から重症化まで多岐にわたっており，宿主の相互作用や感染株の病原性に起因している．本研究では、ヒト感染株3株とブロイラー感染株2株をカテコラミン（エピネフリン、ノルエピネフリン、グルココルチコイド神経内分泌ホルモンであるコルチコステロン、コルチゾール、コルチゾン）に曝露し、成長、運動性、接着性、浸潤性に及ぼす宿主ストレスの影響を調べた。カテコールアミンは、カコ-２細胞の成長、接着、浸潤を有意に増加させる結果となった。コルチコステロンは5系統中1系統で増殖を促進し、コルチゾンは5系統中2系統で運動性を有意に増加させたが、コルチゾールの添加では有意差は認められなかった。以上のことから、ストレス関連ホルモン、特にカテコールアミンが、.NET の成長と病原性を促進する可能性があると結論づけられた。

spp. are major causes of foodborne illness globally, and are mostly transmitted through the consumption and handling of poultry. infections have widely variable outcomes, ranging from mild enteritis to severe illness, which are attributed to host interactions and the virulence of the infecting strain. In this study, in order to investigate the effect of host stress on the growth and pathogenicity of , three strains associated with human infection and two strains from broilers were subject to growth, motility, adhesion and invasion assays, in response to exposure to catecholamines; epinephrine, norepinephrine and the glucocorticoid neuroendocrine hormones corticosterone, cortisol and cortisone which are associated with stress in humans and broilers. Catecholamines resulted in significantly increased growth, adhesion and invasion of Caco-2 cells. Corticosterone promoted growth in one of five strains, and cortisone resulted in a significant increase in motility in two out of five strains, while no significant differences were observed with the addition of cortisol. It was concluded that stress-associated hormones, especially catecholamines, may promote growth and virulence in .