肺局所免疫の確立におけるB7ファミリー共抑制分子の発現

[Expression of B7 Family Co-inhibitory Molecules in the Establishment of Pulmonary Regional Immunity].

• Yu-Qin Xie
• Fang Shi
• Bang-Rong Cao
• Hua-Jing Wang
• Han-Min Liu

抄録

目的:

肺の発生過程におけるB7ファミリー共抑制分子の時間的・空間的発現を明らかにし、肺局所免疫の発生過程におけるB7ファミリー共抑制分子の役割を探る。

OBJECTIVE: To identify the temporal-spatial expression of B7 family co-inhibitory molecules during lung development, and to explore the roles of B7 family co-inhibitory molecules in the developmental process of pulmonary regional immunity.

方法:

アカゲザル肺の異なる発生段階におけるB7ファミリー共抑制分子（B7-1, B7-2, B7-H1, B7-DC）の発現を正規化し、100万個のマップされたリード（Read per kilo-base of transcript per million mapped reads）法（RPKM）により算出した。免疫組織化学染色を行い、マウスの異なる発生段階（カナル期、嚢胞期、肺胞期）におけるB7ファミリー共抑制分子の局在とタンパク質の同定を行った。

METHODS: The expression of B7 family co-inhibitory molecules (B7-1, B7-2, B7-H1, B7-DC) in different developmental stages of Rhesus monkey lungs were normalized and calculated by the reads per kilo-base of transcript per million mapped reads (RPKM) method. Immunohistochemical staining was performed to identify the localization and the protein of B7 family co-inhibitory molecules in different developmental phase (canalicular stage, cystic stage, alveolar stage) in mouse.

結果:

アカゲザルのB7ファミリー共抑制分子の発現は出生前期（嚢胞期、肺胞期）に増加し、肺胞期ではB7ファミリー共抑制分子の発現とmRNAの発現が有意に増加した（<0.05）。免疫組織化学の結果、B7ファミリー共抑制分子は主に気道上皮細胞で発現しており、そのタンパク質量は出生前期に増加していた。B7-2、B7-H1、B7-DCの発現は管腔期から有意に増加した（<0.05）。B7-2の蛋白質は気管支より気道の方が高かった(<0.05)。

RESULTS: The expression of B7 family co-inhibitory molecules in rhesus monkey were increased during the prenatal period (cystic stage, alveolar stage), the expressions of and mRNA were significantly increased in alveolar stage (<0.05). The results of immuno-histochemistry indicated that B7 family co-inhibitory molecules were mainly expressed in airway epithelial cells, and their protein levels were increased during the prenatal period. The expressions of B7-2, B7-H1 and B7-DC were significantly increased from canalicular stage (<0.05). The protein of B7-2 was higher in airway than that in bronchus (<0.05).

結論:

その結果、B7ファミリー共抑制分子は主に気道上皮細胞で発現しており、出生前の時期に発現が増加していることから、B7ファミリー共抑制分子が肺局所免疫の発達に関与している可能性が示唆された。

CONCLUSIONS: B7 family co-inhibitory molecules are mainly expressed in airway epithelial cells, and the expressions are increased during the prenatal period, which suggests that B7 family co-inhibitory molecules are involved possibly in the development of pulmonary regional immunity.

Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).