ウレアプラズマ・ウレアリチカム（UU）感染がラットの精巣組織構造と分泌機能に及ぼす影響

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Sichuan Da Xue Xue Bao Yi Xue Ban.2018 Nov;49(6):895-898.

ウレアプラズマ・ウレアリチカム（UU）感染がラットの精巣組織構造と分泌機能に及ぼす影響

[Influence of Ureaplasma Urealyticum Infection on Testicular Tissue Structure and Secretion Function in Rats].

Tao Zhang
Wen-Xuan Yang
Feng-Ling Yu
Jun-Chang Guan
Lei Zhou
Huai-Yong Gan
Cong-Sen Liu

PMID: 32677400

抄録

目的:

ラットにおける精巣組織の構造と分泌機能に及ぼすウレアプラズマ・ウレアリチカム（UU）感染の影響を検討する。

OBJECTIVE: To study the influences of ureaplasma urealitycum (UU) infection on testicular tissue structure and secretion function in rats.

方法:

クリーングレードの雄性ＳＤラット４０匹を実験群Ａ（術後７日目）、実験群Ｂ（術後１４日目）、対照群Ｃ（術後７日目）、対照群Ｄ（術後１４日目）に無作為に分けた。ラットは各群10匹であった。実験群には膀胱からUU4を0.6mL注入した。対照群にも同様に、同量のUU液培地を注入した。注入後7日目と14日目に、全ラットの精巣の構造を光顕微鏡で観察し、精子形成細胞を透過電子顕微鏡で観察した。血漿中および精巣液中のテストステロンの含有量は化学発光法により検出した。

METHODS: Forty clean grade male SD rats were randomly divided into the experiment group A (at 7 d after surgery), experiment group B (at 14 d after surgery), control group C (at 7 d after surgery) and control group D (at 14 d after surgery). There were 10 rats in each group. The experimental groups were injected with 0.6 mL UU4 through bladder. In the same way, the control groups were injected with the same volume of UU liquid medium. At day 7 and 14 after injection, the structures of testis of all rats were observed by light microscopy and spermatogenic cells by transmission electron microscopy. The content of testosterone in plasma and testicular fluid were detected by chemiluminescence method.

結果:

Ａ群とＢ群の精巣の炎症性病理の変化（造精細胞の減少、炎症性細胞の浸潤、成熟精子の減少など）を光顕微鏡で観察したところ、Ｂ群の炎症性変化はＡ群に比べて軽度であり、Ｃ群とＤ群の精巣組織の構造は正常であった。A群とB群の精巣における生殖細胞のアポトーシス性能（細胞膜の波状化、核クロマチン濃度、核破裂を含む）は透過型電子顕微鏡で確認され、B群の変化はA群に比べて軽度であった。A群とB群の血漿テストステロンのレベルは、C群とD群に比べて有意に低かった（<0.01）が、A群とB群の差は統計的に有意ではなかった。A群の精巣間質液中のテストステロン濃度は、他の群のそれよりも有意に低かった(<0.01)、他の群間の差は統計的に有意ではなかった。

RESULTS: The changes of inflammatory pathology (including the layer and amount of spermatogenic cell decreasing, inflammatory cell infiltrating and mature sperms decreasing) in the testis of group A and group B were found by light microscopy, and the inflammatory changes in group B were lighter than those in group A. The structures of testicular tissue in group C and group D were normal. The apoptosis performances of germ cell (including the cell membrane corrugated, nuclear chromatin concentration and nuclear rupture) in the testis of group A and group B were found by transmission electron microscopy, and the changes in group B were lighter than those in group A. The structures of germ cell in group C and group D were normal. The levels of plasma testosterone in group A and group B were significantly lower than that in group C and group D (<0.01), the difference between group A and group B was not statistically significant. The testosterone level in testis interstitial fluid in group A was significantly lower than that in other groups (<0.01), the differences between other groups were not statistical significant.

結論:

UU感染ラットの精巣組織には様々な病理学的損傷や機能的変化が認められ、UUU感染が男性不妊の原因となることがさらに確認されました。UU感染後のラットの精巣組織の病理学的損傷や機能的変化は、罹病期間の延長に伴って徐々に回復する可能性がある。

CONCLUSIONS: The testicular tissue of UU infected rats can have various pathological damage and functional changes, further confirming that UU infection can cause male infertility. The pathological damage and functional changes of the testicular tissue of rats after UU infection can be gradually restored with the extension of the duration of the disease.