2型糖尿病患者および非糖尿病患者における歯内・歯周病変に関連する3種類の嫌気性細菌のPCR法による検出

PCR-based detection of three anaerobic bacteria associated with endodontic-periodontic lesions in type-2 diabetic and nondiabetic subjects.

抄録

目的:

本研究の目的は、ポリメラーゼ連鎖反応（PCR）を用いて、2型糖尿病患者および非糖尿病患者の歯内・歯周病変に関連する3種類の嫌気性細菌を臨床的に分離・検出することである。

AIM: The aim of this study is to clinically isolate and detect three anaerobic bacteria associated with endodontic-periodontal lesions in type-2 diabetic and nondiabetic patients using polymerase chain reaction (PCR).

材料と方法:

歯内・歯周病変を有する患者60名を2群に分けた。2型糖尿病患者30名（グループ1）と非糖尿病患者30名（グループ2）を対象に、3種類の嫌気性菌の存在を評価した。臨床検査，周囲X線写真，管腔系と歯周ポケットからの微生物学的サンプリングを行った．細菌の定性評価は，Multiplex PCRを用いて行った．統計解析はピアソンのカイ二乗検定およびフィッシャーの正確検定を用いて行った。

MATERIALS AND METHODS: Sixty patients presenting endodontic-periodontal lesions were divided into two groups. Thirty patients with type-2 diabetics (Group 1) and 30 nondiabetic patients (Group 2) were evaluated for the presence of three anaerobic bacteria. Clinical examinations, periapical radiographs, and microbiological sampling from the canal system and periodontal pockets were performed. Qualitative evaluation of bacteria was performed using a multiplex PCR for and . Statistical analysis was performed using Pearson's Chi-square test and Fischer's exact test.

結果:

(2型糖尿病患者の根管から分離された細菌は、（73.3％）が優勢であり、次いで（70％）、（36％）であったのに対し、非糖尿病患者ではそれぞれ53.3％、43.3％、23.3％であった。 (2型糖尿病患者の歯周ポケットから分離された細菌は、（73.3％）が最も多く、次いで（50％）、（30％）であった（非糖尿病患者ではそれぞれ36.6％、33.3％、30％）。

RESULTS: (73.3%) was the predominant bacteria isolated from the root canal in type 2 diabetic patients, followed by (70%) and (36%) compared to 53.3%, 43.3%, and 23.3%, respectively, among nondiabetic patients. (73.3%) was the predominant bacteria isolated from periodontal pockets in type II diabetic patients followed by 50% and 30% compared to 36.6%, 33.3%, and 30%, respectively, among nondiabetics. was detected in the root canal and periodontal pocket in almost similar numbers (70% and 73%), respectively, among type-2 diabetics.

結論:

が根管内および歯周ポケット内の検体から検出されたことから、2つの部位から感染が伝播する可能性が示唆された。Since in the present study, was found to be present in similar numbers in dual sites among type 2 diabetic patients, importance should be given in treating such anaerobic bacteria in immune-compromised patients.

CONCLUSION: Detection of , , and in both root canal and periodontal pocket samples confirm a viable pathway for the spread of infection through dual sites. Since in the present study, was found to be present in similar numbers in dual sites among type 2 diabetic patients, importance should be given in treating such anaerobic bacteria in immune-compromised patients.