口腔白板症の唾液細菌シフトは、dysbiotic oral cancer bacteriomeに類似している

Salivary bacterial shifts in oral leukoplakia resemble the dysbiotic oral cancer bacteriome.

抄録

:口腔癌の中には、自然に発生するものもあるが、悪性の可能性を秘めた口腔の長年の病態の中で発生するものもあり、現在では口腔潜在性悪性疾患（OPMD）として知られている。OPMDに関連する口腔内細菌叢は、口腔癌に関連する細菌叢に比べてあまり研究されていない。白板症患者20名、口腔癌患者31名、健常対照者23名のWMFバクテリオームを、Illumina MiSeqプラットフォームを用いてプロファイリングした。シーケンシングリードはDADA2を用いて処理し、系統的配置法を用いて分類を行った。白板症と口腔癌のWMFバクテリオームにはかなりの重複が認められ、健常者と前2疾患の間にはより明確な分離が認められました。その結果，健常者と口腔癌の分離は，14の属と.口腔白板症と口腔癌では、口腔内細菌が口腔癌の初期発生に関与している可能性が示唆された。この結果は、口腔がん発症の初期段階における微生物ドライバーを標的とした口腔がん予防戦略の開発に示唆を与えるものと思われる。

: While some oral carcinomas appear to arise , others develop within long-standing conditions of the oral cavity that have malignant potential, now known as oral potentially malignant disorders (OPMDs). The oral bacteriome associated with OPMD has been studied to a lesser extent than that associated with oral cancer. To characterize the association in detail we compared the bacteriome in whole mouth fluid (WMF) in patients with oral leukoplakia, oral cancer and healthy controls. : WMF bacteriome from 20 leukoplakia patients, 31 patients with oral cancer and 23 healthy controls were profiled using the Illumina MiSeq platform. Sequencing reads were processed using DADA2, and taxonomical classification was performed using the phylogenetic placement method. Sparse Partial Least Squares Regression Discriminant Analysis model was used to identify bacterial taxa that best discriminate the studied groups. : We found considerable overlap between the WMF bacteriome of leukoplakia and oral cancer while a clearer separation between healthy controls and the former two disorders was observed. Specifically, the separation was attributed to 14 taxa belonging to the genera and . The most discriminative bacterial genera between leukoplakia and oral cancer were : Oral bacteria may play a role in the early stages of oral carcinogenesis as a dysbiotic bacteriome is associated with oral leukoplakia and this resembles that of oral cancer more than healthy controls. Our findings may have implications for developing oral cancer prevention strategies targeting early microbial drivers of oral carcinogenesis.