フッ化ナトリウムとキシリトールを含有する塩化セチルピリジニウム系洗口剤の抗菌活性、生体適合性および抗炎症性.In Vitro試験

Antimicrobial Activity, Biocompatibility and Anti-inflammatory Properties of Cetylpyridinium Chloride-based Mouthwash Containing Sodium Fluoride and Xylitol: An In Vitro Study.

• Geneviève LeBel
• Katy Vaillancourt
• Marie-Pierre Morin
• Daniel Grenier

抄録

目的:

機械的なプラーク除去の補助としての洗口剤の使用は、口腔衛生の改善に役立つ可能性がある。本研究では、フッ化ナトリウムとキシリトールを配合したセチルピリジニウムクロライド（CPC）ベースの洗口剤（X-PUR Opti-Rinse 0.05% NaFとX-PUR Opti-Rinse 0.2% NaF）を用いて、虫歯、歯周病、カンジダ症に関連する重要な口腔内病原体に対する抗菌活性を評価した。また、その際には、「お風呂に入っているときには、お風呂に入っているときには、お風呂に入っているときには、お風呂に入っていないでください。微生物学的特性は、プランクトンおよびバイオフィルム培養物の両方に対して評価した。<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br>1.CPCベースの洗口剤は、S. mutans、S. sobrinus、P. gingivalisのバイオフィルムに対しても殺菌効果を示した。口腔上皮細胞モデルを用いて、CPCベースのマウスウォッシュは、対照として使用したクロルヘキシジン含有マウスウォッシュよりも細胞毒性が低いことが判明した。<br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br /> <br腸内環境を改善するためには、口腔内環境を改善することが重要であると考えられます。さらに、口腔上皮細胞モデルを用いて、これらの洗口剤は生体適合性が高く、抗炎症活性を示すことが明らかになった。

PURPOSE: The use of a mouthwash as an adjunct to mechanical plaque removal may be useful to improve oral hygiene. In this study, cetylpyridinium chloride (CPC)-based mouthwashes containing sodium fluoride and xylitol (X-PUR Opti-Rinse 0.05% NaF and X-PUR Opti-Rinse 0.2% NaF) were evaluated for their antimicrobial activity against important oral pathogens associated with dental caries, periodontal disease, and candidiasis. Moreover, their biocompatibility and anti-inflammatory properties were assessed.<br />Materials and Methods: Antimicrobial activity was determined using a disk-diffusion assay, a microplate dilution assay, and the European standard protocols for antiseptics. Microbicidal properties were assessed against both planktonic and biofilm cultures. An oral epithelial cell model was used to evaluate the biocompatibility of mouthwashes and their ability to attenuate cytokine secretion.<br />Results: Using three different antimicrobial assays, the CPC-based mouthwashes were found to be highly active against the tested microorganisms. More specifically, the mouthwashes met the European Standard NF EN 1040 and NF EN 1275 defined as a log10 reduction ≥ 5 (≥ 99.999% killing) for bacteria and log10 reduction ≥ 4 (≥ 99.99% killing) for fungi, respectively. The CPC-based mouthwashes were also bactericidal against biofilms of S. mutans, S. sobrinus, and P. gingivalis. Using an oral epithelial cell model, the CPC-based mouthwashes were found to be less cytotoxic than a chlorhexidine-containing mouthwash used as control. Lastly, the CPC-based mouthwashes decreased the secretion of interleukin-6 and interleukin-8 by lipopolysaccharide-stimulated oral epithelial cells. <br />Conclusion: The CPC-based mouthwashes supplemented with sodium fluoride (0.05% or 0.2%) and xylitol (10%) were highly active against important oral pathogens. Moreover, using an oral epithelial cell model, these mouthwashes were found to be biocompatible and to exhibit anti-inflammatory activity.