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くさび形欠損と無傷の歯を持つ患者のエナメル質の化学組成と口腔液の相関関係について
THE CORRELATION OF THE CHEMICAL COMPOSITION OF ENAMEL AND ORAL FLUID IN PATIENTS WITH A WEDGE-SHAPED DEFECT AND INTACT TEETH.
PMID: 33526727
抄録
目的 - くさび形欠損症と無傷の硬組織を持つ患者の口腔液の化学組成とミネラル化レベルを決定し、頸部エナメル質と口腔液の指標の相関を実施する。口腔液中のСa2+, РО43-, SO42を分光光度法で、Na+, К+, Al3+を原子吸光法で、Mg2+, Zn2+を原子吸光法で、Cl-をpH計の塩化物選択電極で測定した。抜歯した22本の歯(12本は無傷の歯、10本はウェッジシェイプ)の口腔液と歯頸部エナメル質の化学組成の相関を調べた。JSM-6490 LV集束ビーム電子顕微鏡(走査型)とエネルギー分散型X線マイクロアナリシスINCA Penta FETх3を使用した。 口腔液中の化学分析物の含有量とモル比には有意な差は見られなかった(p>0.05)。対照群の患者では、鉱化能のレベルが1.64+/-0.11倍高かった(p≦0.05)。くさび形欠損のある歯のエナメル質のモル係数の値が高かった。Mg/Рが1.63倍、Ca/Pが24%と高かったが、低かった。Mg/Саは4.7倍、Р/Саは14%であった(p≤0.05)。対照群の患者の口腔液とエナメル質の化学組成の相関関係の違いは、平均的な正比例の結合強度の存在であった。Na+とCl-およびMg2+、反比例している。Na+とСa2+およびРО43-, р<0.0001.対照群の口腔液とエナメル質のモル係数の相関は、高い相関と中程度の相関があることで区別された。直接的に比例するもの-Са2+/Cl-とNa+/К+, Са2+/Mg2+; Mg2+/Са2+とРО43-/Са2+、反比例-Са2+/Mg2+とРО43-/Са2+の間、р<0.0001.歯のエナメル質のミネラル状態の評価や、混合唾液の力学的観点から楔状欠損の初期形態の予防効果をコントロールするために利用可能な、エナメル質と口腔液の化学組成の相関関係を明らかにしました。
Aim - to determine oral fluid chemical composition and mineralization level in patients with a wedge-shaped defect and intact hard tissues, conduct correlation between the indicators of cervical enamel and oral fluid. We determined Сa2+, РО43-, SO42 using spectrophotometric method, Na+, К+, Al3+ by atomic emission spectrometry, Mg2+, Zn2+ by atomic absorption spectrometry, Cl- using chloride-selective electrode of pH-meter in oral fluid of 22 patients divided into two groups: case - with wedge-shaped defects (2.81+/- 0.73); control - with intact teeth. Correlation of oral fluid and cervical enamel chemical composition of 22 extracted teeth (12 - intact teeth, 10 wedge shape) was made. We used JSM-6490 LV focused beam electron microscope (scanning) with system of energy-dispersive X-ray microanalysis INCA Penta FETх3. Significant differences haven't been found in chemical analyte content and molar ratios (p>0.05) in oral fluid. The level of mineralization potential was 1.64+/-0.11 times higher in patients of the control group (p≤0.05). The values of molar coefficients in teeth enamel with a wedge-shaped defect were high: Mg/Р by 1.63 times, Ca/P by 24%, they were low: Mg/Са - by 4.7 times, Р/Са - by 14% (p≤0.05). The difference in correlation between the chemical composition of oral fluid and enamel of patients in the control group was the presence of an average directly proportional bond strength: Na+ with Cl- and Mg2+, inversely proportional: Na+ with Сa2+ and РО43-, р<0.0001. Correlation between molar coefficients of oral fluid and enamel in the control group was distinguished by the presence of high and moderate correlation: directly proportional - between Са2+/Cl- and Na+/К+, Са2+/Mg2+; Mg2+/Са2+ and РО43-/Са2+, inversely proportional - between Са2+/Mg2+ and РО43-/Са2+, р<0.0001. We have revealed correlations of the chemical composition of enamel and oral fluid which can be used to assess the mineral state of tooth enamel and control the effectiveness of prevention of the initial forms of wedge-shaped defects in terms of mixed saliva in dynamics.